Carvacrol and Thymol Attenuate Cytotoxicity Induced by Amyloid β25-35 via Activating Protein Kinase C and Inhibiting Oxidative Stress in PC12 Cells.
Amyloid beta-Peptides
/ toxicity
Animals
Apoptosis
/ drug effects
Cell Survival
/ drug effects
Cymenes
/ adverse effects
Enzyme Activation
/ drug effects
Neuroprotective Agents
/ pharmacology
Oxidative Stress
/ drug effects
PC12 Cells
Peptide Fragments
/ toxicity
Protein Kinase C
/ metabolism
Rats
Reactive Oxygen Species
/ metabolism
Thymol
/ adverse effects
Alzheimer’s disease
Carvacrol
Reactive oxygen species
Thymol
Journal
Iranian biomedical journal
ISSN: 2008-823X
Titre abrégé: Iran Biomed J
Pays: Iran
ID NLM: 9814853
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
entrez:
21
4
2020
pubmed:
21
4
2020
medline:
5
2
2021
Statut:
ppublish
Résumé
Our previous findings indicated that carvacrol and thymol alleviate cognitive impairments caused by Aβ in rodent models of Alzheimer's disease (AD). In this study, the neuroprotective effects of carvacrol and thymol against Aβ25-35-induced cytotoxicity were evaluated, and the potential mechanisms were determined. PC12 cells were pretreated with Aβ25-35 for 2 h, followed by incubation with carvacrol or thymol for additional 48 h. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. A flurospectrophotometer was employed to observe the intracellular reactive oxygen species (ROS) production. Protein kinase C (PKC) activity was analyzed using ELISA. Our results indicated that carvacrol and thymol could significantly protect PC12 cells against Aβ25-35-induced cytotoxicity. Furthermore, Aβ25-35 could induce intracellular ROS production, while carvacrol and thymol could reverse this effect. Moreover, our findings showed that carvacrol and thymol elevate PKC activity similar to Bryostatin-1, as a PKC activator. This study provided the evidence regarding the protective effects of carvacrol and thymol against Aβ25–35-induced cytotoxicity in PC12 cells. The results suggested that the neuroprotective effects of these compounds against Aβ25-35 might be through attenuating oxidative damage and increasing the activity of PKC as a memory-related protein. Thus, carvacrol and thymol were found to have therapeutic potential in preventing or modulating AD.
Sections du résumé
Background
Our previous findings indicated that carvacrol and thymol alleviate cognitive impairments caused by Aβ in rodent models of Alzheimer's disease (AD). In this study, the neuroprotective effects of carvacrol and thymol against Aβ25-35-induced cytotoxicity were evaluated, and the potential mechanisms were determined.
Methods
PC12 cells were pretreated with Aβ25-35 for 2 h, followed by incubation with carvacrol or thymol for additional 48 h. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. A flurospectrophotometer was employed to observe the intracellular reactive oxygen species (ROS) production. Protein kinase C (PKC) activity was analyzed using ELISA.
Results
Our results indicated that carvacrol and thymol could significantly protect PC12 cells against Aβ25-35-induced cytotoxicity. Furthermore, Aβ25-35 could induce intracellular ROS production, while carvacrol and thymol could reverse this effect. Moreover, our findings showed that carvacrol and thymol elevate PKC activity similar to Bryostatin-1, as a PKC activator.
Conclusion
This study provided the evidence regarding the protective effects of carvacrol and thymol against Aβ25–35-induced cytotoxicity in PC12 cells. The results suggested that the neuroprotective effects of these compounds against Aβ25-35 might be through attenuating oxidative damage and increasing the activity of PKC as a memory-related protein. Thus, carvacrol and thymol were found to have therapeutic potential in preventing or modulating AD.
Identifiants
pubmed: 32306722
doi: 10.29252/ibj.24.4.243
pmc: PMC7275817
doi:
Substances chimiques
Amyloid beta-Peptides
0
Cymenes
0
Neuroprotective Agents
0
Peptide Fragments
0
Reactive Oxygen Species
0
amyloid beta-protein (25-35)
0
Thymol
3J50XA376E
carvacrol
9B1J4V995Q
Protein Kinase C
EC 2.7.11.13
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
243-50Références
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