Improving the Catalytic Performance of Pectate Lyase Through Pectate Lyase/Cu
enzyme immobilization
hybrid nanoflowers
pectate lyases
textile industry
thermostability
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
01
2020
accepted:
17
03
2020
entrez:
21
4
2020
pubmed:
21
4
2020
medline:
21
4
2020
Statut:
epublish
Résumé
Pectate lyases (Pels) can be used in the textile industrial process for cotton scouring and ramie degumming, and its hydrolyzed products oligo galacturonic acid, are high-value added agricultural and health products. In our previous studies, an alkaline pectate lyase PEL168 mutant, PEL3, was obtained with improved specific activity and thermostability. Here, a facile and rapid method for preparing an immobilized PEL3-inorganic hybrid nanoflower was developed, as it could improve its biocatalytic performance. With 0.02 mg/mL (112.2 U/mL) PEL3 in PBS buffer, five different divalent ions, including Mn
Identifiants
pubmed: 32309279
doi: 10.3389/fbioe.2020.00280
pmc: PMC7145898
doi:
Types de publication
Journal Article
Langues
eng
Pagination
280Informations de copyright
Copyright © 2020 Wu, Luo, Lu, Zhan and Zhang.
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