Survival of Rickettsia conorii in artificially contaminated whole and leukoreduced canine blood units during the storage period.
Blood transfusion
Dog
Leukoreduction
Rickettsia conorii
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Apr 2020
21 Apr 2020
Historique:
received:
15
12
2019
accepted:
24
02
2020
entrez:
22
4
2020
pubmed:
22
4
2020
medline:
31
12
2020
Statut:
epublish
Résumé
The ability of tick-borne agents to survive in stored blood bags is a key factor for their transmissibility by blood transfusion. The aim of this study was to evaluate the survival and potential infectivity of Rickettsia conorii (RC) in artificially contaminated canine whole blood (WB) and in leukoreduced whole blood (LR-WB) during the storage period. RC was cultured on L929 cells. We used a one-week 25-cm RC survived for the entire storage period in both whole and leukoreduced blood. All bags contained viable bacteria until 7 dpi; RC viability generally decreased over time, particularly in LR-WB bags where the isolation time was longer than in WB. Viable bacteria were still isolated at 35 dpi in 3 WB and 3 LR-WB. Leukoreduction reduced but did not eliminate RC in infected units. The survival and infectivity of RC in canine blood during the storage period may represent a threat for recipients.
Sections du résumé
BACKGROUND
BACKGROUND
The ability of tick-borne agents to survive in stored blood bags is a key factor for their transmissibility by blood transfusion. The aim of this study was to evaluate the survival and potential infectivity of Rickettsia conorii (RC) in artificially contaminated canine whole blood (WB) and in leukoreduced whole blood (LR-WB) during the storage period.
METHODS
METHODS
RC was cultured on L929 cells. We used a one-week 25-cm
RESULTS
RESULTS
RC survived for the entire storage period in both whole and leukoreduced blood. All bags contained viable bacteria until 7 dpi; RC viability generally decreased over time, particularly in LR-WB bags where the isolation time was longer than in WB. Viable bacteria were still isolated at 35 dpi in 3 WB and 3 LR-WB.
CONCLUSIONS
CONCLUSIONS
Leukoreduction reduced but did not eliminate RC in infected units. The survival and infectivity of RC in canine blood during the storage period may represent a threat for recipients.
Identifiants
pubmed: 32312308
doi: 10.1186/s13071-020-3991-9
pii: 10.1186/s13071-020-3991-9
pmc: PMC7171768
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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