A molecular survey of vector-borne pathogens and haemoplasmas in owned cats across Italy.
Age Factors
Anaplasma
/ isolation & purification
Animals
Babesia
/ isolation & purification
Bartonella
/ isolation & purification
Cat Diseases
/ epidemiology
Cats
/ microbiology
DNA, Bacterial
/ genetics
Ehrlichia
/ isolation & purification
Female
Geography
Italy
/ epidemiology
Male
Mycoplasma
/ classification
Mycoplasma Infections
/ blood
Pets
/ microbiology
Prevalence
Sex Factors
Bartonella spp.
Cat
Feline immunodeficiency virus
Feline leukemia virus
Haemoplasmas
Leishmania infantum
Vector-borne pathogens
Zoonosis
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
21 Apr 2020
21 Apr 2020
Historique:
received:
09
01
2020
accepted:
24
02
2020
entrez:
22
4
2020
pubmed:
22
4
2020
medline:
31
12
2020
Statut:
epublish
Résumé
Feline vector-borne pathogens (FeVBPs) have been increasingly investigated for their impact on cat health and their zoonotic potential. The aim of the present study was to assess the prevalence of FeVBPs and haemoplasmas in cats across Italy and to identify potential risk factors linked to their occurrence. Blood samples from 958 owned cats living in the North (n = 556), Centre (n = 173) and South (n = 229) of Italy were tested for Babesia spp., Hepatozoon spp., Ehrlichia spp., Anaplasma spp. and filarioids by conventional PCR (cPCR) and for haemoplasmas and Bartonella spp. by SYBR green real-time PCR. Cats included in the study represent a sub-sample from a larger number of animals enrolled in a previous study, which were selected based on the geographical origin. Data on cats' positivity for Leishmania infantum, feline leukaemia virus (FeLV) and for feline immunodeficiency virus (FIV), available from the previous study, were included and examined. Potential risk factors for pathogen infection were assessed in relationship to categorical variables including sex, geographical origin, breed, neutering status and age of cats. Out of the 958 cats, 194 (20.2%) were positive for at least one of the tested pathogens, 89 (16%) from the North, 32 (18.5%) from the Centre and 73 (31.9%) from the South of Italy. A high prevalence of FeVBPs was detected in male cats (n = 125, 27.8%), living in the southern part of the country (n = 73, 31.9%), younger than 18 months of age (n = 24, 22.4%) and not neutered (n = 39; 27.5%). In particular, 24 cats (2.5%) tested PCR-positive for Bartonella spp., of which 1.6% for B. henselae and 0.9% for B. clarridgeiae. A total of 111 cats scored PCR-positive for haemoplasmas (11.6%), specifically "Candidatus Mycoplasma haemominutum" (n = 95, 9.9%), M. haemofelis (n = 14, 1.5%) and "Candidatus Mycoplasma turicensis" (n = 2, 0.2%). Moreover, 39, 31 and 8 cats were positive for FeLV (4.1%), L. infantum (3.2%) and FIV (0.8%), respectively. Co-infections were registered for 19 (9.8%) cats. These results confirm the occurrence of haemoplasmas and FeVBPs throughout Italy. Preventive measures to protect both animal and human health should be carried out also for owned cats, even if no health status of animals has been assessed in this study.
Sections du résumé
BACKGROUND
BACKGROUND
Feline vector-borne pathogens (FeVBPs) have been increasingly investigated for their impact on cat health and their zoonotic potential. The aim of the present study was to assess the prevalence of FeVBPs and haemoplasmas in cats across Italy and to identify potential risk factors linked to their occurrence.
METHODS
METHODS
Blood samples from 958 owned cats living in the North (n = 556), Centre (n = 173) and South (n = 229) of Italy were tested for Babesia spp., Hepatozoon spp., Ehrlichia spp., Anaplasma spp. and filarioids by conventional PCR (cPCR) and for haemoplasmas and Bartonella spp. by SYBR green real-time PCR. Cats included in the study represent a sub-sample from a larger number of animals enrolled in a previous study, which were selected based on the geographical origin. Data on cats' positivity for Leishmania infantum, feline leukaemia virus (FeLV) and for feline immunodeficiency virus (FIV), available from the previous study, were included and examined. Potential risk factors for pathogen infection were assessed in relationship to categorical variables including sex, geographical origin, breed, neutering status and age of cats.
RESULTS
RESULTS
Out of the 958 cats, 194 (20.2%) were positive for at least one of the tested pathogens, 89 (16%) from the North, 32 (18.5%) from the Centre and 73 (31.9%) from the South of Italy. A high prevalence of FeVBPs was detected in male cats (n = 125, 27.8%), living in the southern part of the country (n = 73, 31.9%), younger than 18 months of age (n = 24, 22.4%) and not neutered (n = 39; 27.5%). In particular, 24 cats (2.5%) tested PCR-positive for Bartonella spp., of which 1.6% for B. henselae and 0.9% for B. clarridgeiae. A total of 111 cats scored PCR-positive for haemoplasmas (11.6%), specifically "Candidatus Mycoplasma haemominutum" (n = 95, 9.9%), M. haemofelis (n = 14, 1.5%) and "Candidatus Mycoplasma turicensis" (n = 2, 0.2%). Moreover, 39, 31 and 8 cats were positive for FeLV (4.1%), L. infantum (3.2%) and FIV (0.8%), respectively. Co-infections were registered for 19 (9.8%) cats.
CONCLUSIONS
CONCLUSIONS
These results confirm the occurrence of haemoplasmas and FeVBPs throughout Italy. Preventive measures to protect both animal and human health should be carried out also for owned cats, even if no health status of animals has been assessed in this study.
Identifiants
pubmed: 32312323
doi: 10.1186/s13071-020-3990-x
pii: 10.1186/s13071-020-3990-x
pmc: PMC7171850
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116Références
J Clin Microbiol. 2007 Apr;45(4):1159-66
pubmed: 17301277
Vet Microbiol. 2006 Dec 20;118(3-4):274-7
pubmed: 16919405
Biomed Res Int. 2014;2014:943754
pubmed: 24895629
Parasit Vectors. 2013 Apr 15;6:99
pubmed: 23587366
JFMS Open Rep. 2018 May 22;4(1):2055116918770037
pubmed: 29854412
J Clin Microbiol. 1995 Sep;33(9):2445-50
pubmed: 7494043
J Feline Med Surg. 2013 Oct;15(10):879-85
pubmed: 23482254
J Feline Med Surg. 2013 Jul;15(7):563-9
pubmed: 23813816
Trends Parasitol. 2017 Oct;33(10):813-825
pubmed: 28648798
Vet Parasitol. 2018 Feb 15;251:131-137
pubmed: 29426470
J Feline Med Surg. 2009 Apr;11(4):277-85
pubmed: 18790658
Parasit Vectors. 2018 Mar 20;11(1):136
pubmed: 29554931
Rev Inst Med Trop Sao Paulo. 2014 Mar-Apr;56(2):93-5
pubmed: 24626408
Comp Immunol Microbiol Infect Dis. 2019 Oct;66:101344
pubmed: 31437677
Ticks Tick Borne Dis. 2017 Aug;8(5):721-724
pubmed: 28576619
Vet Clin North Am Small Anim Pract. 2010 Nov;40(6):1157-70
pubmed: 20933142
Vet Parasitol. 2007 Apr 30;145(3-4):357-60
pubmed: 17174035
Emerg Infect Dis. 2008 Dec;14(12):1922-4
pubmed: 19046522
Int J Antimicrob Agents. 2014 Jul;44(1):16-25
pubmed: 24933445
Vet Parasitol. 2017 Mar 15;236:144-151
pubmed: 28288759
Tierarztl Prax Ausg K Kleintiere Heimtiere. 2017 Oct 17;45(5):329-335
pubmed: 28905982
J Clin Microbiol. 2006 Mar;44(3):961-9
pubmed: 16517884
J Am Vet Med Assoc. 2004 Apr 15;224(8):1270-9
pubmed: 15112775
Parasit Vectors. 2017 Mar 13;10(1):130
pubmed: 28285597
J Clin Microbiol. 1999 Jun;37(6):1782-9
pubmed: 10325324
Comp Immunol Microbiol Infect Dis. 2015 Oct;42:33-6
pubmed: 26387062
Parasit Vectors. 2017 Mar 13;10(1):132
pubmed: 28285588
Vet Parasitol. 2011 Dec 15;182(2-4):221-9
pubmed: 21705146
Clin Microbiol Infect. 2009 Dec;15 Suppl 2:1-3
pubmed: 19416279
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
PLoS Negl Trop Dis. 2019 Jul 15;13(7):e0007594
pubmed: 31306417
J Clin Microbiol. 2004 Jan;42(1):264-8
pubmed: 14715763
Parasit Vectors. 2019 May 2;12(1):193
pubmed: 31046822
J Vet Diagn Invest. 2010 Mar;22(2):270-4
pubmed: 20224091
Exp Parasitol. 2005 Mar;109(3):176-80
pubmed: 15713449
Parasit Vectors. 2010 Jan 11;3:2
pubmed: 20145730
Vector Borne Zoonotic Dis. 2012 Feb;12(2):143-50
pubmed: 22022820
Emerg Infect Dis. 2006 Mar;12(3):389-94
pubmed: 16704774
Vet Parasitol. 2008 Feb 14;151(2-4):332-6
pubmed: 18079064
Berl Munch Tierarztl Wochenschr. 2012 Mar-Apr;125(3-4):163-7
pubmed: 22515036
J Feline Med Surg. 2017 May;19(5):542-548
pubmed: 28438088
Vet Parasitol. 2019 Mar;267:26-31
pubmed: 30878081
Vet Parasitol. 2018 Apr 30;254:26-29
pubmed: 29657007
Vet Parasitol. 2010 Dec 15;174(3-4):336-40
pubmed: 20869810
Parasit Vectors. 2014 Mar 24;7:115
pubmed: 24655431
J Feline Med Surg. 2018 Mar;20(3):256-261
pubmed: 29478400
Vector Borne Zoonotic Dis. 2015 Sep;15(9):529-34
pubmed: 26378972
Vet Parasitol. 2012 Jun 8;187(1-2):302-6
pubmed: 22285010
Parasit Vectors. 2015 Jun 04;8:302
pubmed: 26041555
Parasit Vectors. 2016 May 10;9(1):247
pubmed: 27160725
Parasit Vectors. 2017 Mar 13;10(1):112
pubmed: 28285590
PLoS Negl Trop Dis. 2017 Jan 31;11(1):e0005335
pubmed: 28141857
ISRN Microbiol. 2014 Feb 23;2014:298352
pubmed: 24707436
J Clin Microbiol. 2009 Dec;47(12):4049-54
pubmed: 19828748