Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
Animals
Biological Assay
/ methods
Bone Marrow Cells
/ cytology
Cell Line, Tumor
Colloids
/ chemistry
Dendritic Cells
/ cytology
Female
Flow Cytometry
Fluorescence
Fluorescent Dyes
/ chemistry
Humans
Immunoassay
Lipopolysaccharides
/ pharmacology
Mice, Inbred C57BL
Microspheres
Nanoparticles
/ chemistry
Proteomics
Reference Standards
Journal
Nature biomedical engineering
ISSN: 2157-846X
Titre abrégé: Nat Biomed Eng
Pays: England
ID NLM: 101696896
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
16
01
2019
accepted:
09
03
2020
pubmed:
22
4
2020
medline:
22
7
2020
entrez:
22
4
2020
Statut:
ppublish
Résumé
The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging. Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices. The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element. Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition. It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
Identifiants
pubmed: 32313101
doi: 10.1038/s41551-020-0547-4
pii: 10.1038/s41551-020-0547-4
pmc: PMC7231648
mid: NIHMS1574210
doi:
Substances chimiques
Colloids
0
Fluorescent Dyes
0
Lipopolysaccharides
0
Banques de données
figshare
['10.6084/m9.figshare.11888055']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
518-530Subventions
Organisme : NCI NIH HHS
ID : R01 CA141521
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE027098
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA199092
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002345
Pays : United States
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