Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture.
Compound testing
Hair growth
Human hair follicle
Human scalp skin
Organ culture
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
22
4
2020
pubmed:
22
4
2020
medline:
16
3
2021
Statut:
ppublish
Résumé
The culture of microdissected hair follicles (HFs) and scalp skin enriched in terminal HFs are the best currently available preclinical assays for studying hair and skin biology/pathology in the human system. While microdissected HF organ culture only allows the testing of compounds added into the culture medium, mimicking a systemic application, the scalp skin organ culture also is suitable to test topical and intradermal applications. Here, we describe different methods for isolation of human scalp HFs, the procedures for culturing the scalp skin and microdissected HFs and we also outline different delivery techniques (e.g., topical, systemic) to test active and control substances.
Identifiants
pubmed: 32314211
doi: 10.1007/978-1-0716-0648-3_9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105-119Références
Philpott MP, Green MR, Kealey T (1989) Studies on the biochemistry and morphology of freshly isolated and maintained rat hair follicles. J Cell Sci 93:409–418
doi: 10.1242/jcs.93.3.409
Philpott MP, Green MR, Kealey T (1990) Human hair growth in vitro. J Cell Sci 97:463–471
doi: 10.1242/jcs.97.3.463
Trotter M (1924) The life cycles of hair in selected regions of the body. Am J Phys Anthropol VII:427–437
doi: 10.1002/ajpa.1330070422
Myers RJ, Hamilton JB (1951) Regeneration and rate of growth of hairs in man. Ann N Y Acad Sci 53:562–568
doi: 10.1111/j.1749-6632.1951.tb31957.x
Kloepper JE, Sugawara K, Al-Nuaimi Y et al (2010) Methods in hair research: how to objectively distinguish between anagen and catagen in human hair follicle organ culture. Exp Dermatol 19:305–312
doi: 10.1111/j.1600-0625.2009.00939.x
Langan E, Philpott MP, Kloepper JE et al (2015) Human hair follicle organ culture: theory, application and perspectives. Exp Dermatol 24(12):903–911
doi: 10.1111/exd.12836
Buscone S, Mardaryev AN, Raafs B, Bikker JW, Sticht C, Gretz N, Farjo N, Uzunbajakava NE, Botchkareva NV (2017) A new path in defining light parameters for hair growth: discovery and modulation of photoreceptors in human hair follicle. Lasers Surg Med 49(7):705–718
doi: 10.1002/lsm.22673
Fischer TW, Hipler UC, Elsner P (2007) Effect of caffeine and testosterone on the proliferation of human hair follicles in vitro. Int J Dermatol 46(1):27–35
doi: 10.1111/j.1365-4632.2007.03119.x
Oh JW, Kloepper J, Langan EA, Kim Y, Yeo J, Kim MJ, Hsi TC, Rose C, Yoon GS, Lee SJ, Seykora J, Kim JC, Sung YK, Kim M, Paus R, Plikus MV (2016) A guide to studying human hair follicle cycling in vivo. J Invest Dermatol 136(1):34–44
doi: 10.1038/JID.2015.354
Philpott MP, Sanders DA, Kealey T (1994) Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-I at physiologic concentrations is an important regulator of hair follicle growth in vitro. J Invest Dermatol 102(6):857–861
doi: 10.1111/1523-1747.ep12382494
Chéret J, Bertolini M, Ponce L, Lehmann J, Tsai T, Alam M, Hatt H, Paus R (2018) Olfactory receptor OR2AT4 regulates human hair growth. Nat Commun 9(1):3624
doi: 10.1038/s41467-018-05973-0
Hawkshaw NJ, Hardman JA, Haslam IS, Shahmalak A, Gilhar A, Lim X, Paus R (2018) Identifying novel strategies for treating human hair loss disorders: Cyclosporine A suppresses the Wnt inhibitor, SFRP1, in the dermal papilla of human scalp hair follicles. PLoS Biol 16(5):e2003705
doi: 10.1371/journal.pbio.2003705
Oláh A, Gherardini J, Bertolini M et al (2016) The thyroid hormone analogue KB2115 (eprotirome) prolongs human hair growth (anagen) ex vivo. J Invest Dermatol 36(8):1711–1714
doi: 10.1016/j.jid.2016.03.033
Harel S, Higgins CA, Cerise JE, Dai Z, Chen JC, Clynes R, Christiano AM (2015) Pharmacologic inhibition of JAK-STAT signaling promotes hair growth. Sci Adv 1(9):e1500973
doi: 10.1126/sciadv.1500973
Imanishi H, Ansell DM, Chéret J et al (2018) Epithelial-to-mesenchymal stem cell transition in a human organ: lessons from lichen Planopilaris. J Invest Dermatol 38(3):511–519
doi: 10.1016/j.jid.2017.09.047
Alam A, Hernandez I, Paus R et al (2018) Image gallery: intravital visualization of the dynamic changes in human hair follicle cycling. Br J Dermatol 178(6):e396
doi: 10.1111/bjd.16569
Lu Z, Hasse S, Bodo E et al (2007) Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions. Exp Dermatol 16(1):37–44
doi: 10.1111/j.1600-0625.2006.00510.x
Haslam I, Hardman JA, Paus R (2018) Topically applied nicotinamide inhibits human hair follicle growth ex vivo. J Invest Dermatol 138(6):1420–1422
doi: 10.1016/j.jid.2017.12.019
Gherardini J, Wegner J, Chéret J et al (2019) Transepidermal UV radiation of scalp skin ex vivo induces hair follicle damage that is alleviated by the topical treatment with caffeine. Int J Cosmet Sci 41(2):164–182
doi: 10.1111/ics.12521
Ito T, Ito N, Bettermann A, Tokura Y, Takigawa M, Paus R (2004) Collapse and restoration of MHC class-I-dependent immune privilege: exploiting the human hair follicle as a model. Am J Pathol 164(2):623–634
doi: 10.1016/S0002-9440(10)63151-3
Meyer KC, Bodó E, Brzoska T, Abels C, Paus R (2009) Immunomodulatory effects of the alpha-melanocyte-stimulating hormone-related tripeptide K(D)PT on human scalp hair follicles under proinflammatory conditions. Br J Dermatol 161(6):1400–1403
doi: 10.1111/j.1365-2133.2009.09427.x
Kinori M, Bertolini M, Funk W, Samuelov L, Meyer KC, Emelianov VU, Hasse S, Paus R (2012) Calcitonin gene-related peptide (CGRP) may award relative protection from interferon-γ-induced collapse of human hair follicle immune privilege. Exp Dermatol 21(3):223–226
doi: 10.1111/j.1600-0625.2011.01432.x
Bertolini M, Pretzlaff M, Sulk M, Bahr M, Gherardini J, Uchida Y et al (2016) Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175(3):531–541
doi: 10.1111/bjd.14645
Hardman JA, Tobin DJ, Haslam IS, Farjo N, Farjo B, Al-Nuaimi Y, Grimaldi B, Paus R (2015) The peripheral clock regulates human pigmentation. J Invest Dermatol 135(4):1053–1064
doi: 10.1038/jid.2014.442
Al-Nuaimi Y, Hardman JA, Bíró T, Haslam IS, Philpott MP, Tóth BI, Farjo N, Farjo B, Baier G, Watson REB, Grimaldi B, Kloepper JE, Paus R (2014) A meeting of two chronobiological systems: circadian proteins Period1 and BMAL1 modulate the human hair cycle clock. J Invest Dermatol 134(3):610–619
doi: 10.1038/jid.2013.366
Samuelov L, Sprecher E, Sugawara K, Singh SK, Tobin DJ, Tsuruta D, Bíró T, Kloepper JE, Paus R (2013) Topobiology of human pigmentation: P-cadherin selectively stimulates hair follicle melanogenesis. J Invest Dermatol 133(6):1591–1600
doi: 10.1038/jid.2013.18
Samuelov L, Sprecher E, Tsuruta D, Bíró T, Kloepper JE, Paus R (2012) P-cadherin regulates human hair growth and cycling via canonical Wnt signaling and transforming growth factor-β2. J Invest Dermatol 132(10):2332–2341
doi: 10.1038/jid.2012.171
Paus R, Cotsarelis G (1999) The biology of hair follicles. N Engl J Med 341(7):491–497
doi: 10.1056/NEJM199908123410706
Stenn KS, Paus R (2001) Controls of hair follicle cycling. Physiol Rev 81(1):449–494
doi: 10.1152/physrev.2001.81.1.449
Schneider MR, Schmidt-Ullrich R, Paus R (2009) The hair follicle as a dynamic miniorgan. Curr Biol 19(3):R132–R142
doi: 10.1016/j.cub.2008.12.005
Magerl M, Paus R, Farjo N, Müller-Röver S, Peters EM, Foitzik K, Tobin DJ (2004) Limitations of human occipital scalp hair follicle organ culture for studying the effects of minoxidil as a hair growth enhancer. Exp Dermatol 13(10):635–642
doi: 10.1111/j.0906-6705.2004.00207.x
Gáspár E, Hardenbicker C, Bodó E, Wenzel B, Ramot Y, Funk W, Kromminga A, Paus R (2010) Thyrotropin releasing hormone (TRH): a new player in human hair-growth control. FASEB J 24(2):393–403
doi: 10.1096/fj.08-126417
Ito T, Ito N, Saathoff M, Bettermann A, Takigawa M, Paus R (2005) Interferon-gamma is a potent inducer of catagen-like changes in cultured human anagen hair follicles. Br J Dermatol 152(4):623–631
doi: 10.1111/j.1365-2133.2005.06453.x