Human Wound Healing Ex Vivo Model with Focus on Molecular Markers.


Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 22 4 2020
pubmed: 22 4 2020
medline: 16 3 2021
Statut: ppublish

Résumé

Wound healing is a complex, multifactorial process that is divided in sequential and overlapping phases in order to restore the skin barrier. For the study of wound healing, different in vivo, in vitro, and ex vivo models have been used in the past. Here we describe in detail the methodology of the human skin punch-in-a-punch ex vivo wound healing model.

Identifiants

pubmed: 32314223
doi: 10.1007/978-1-0716-0648-3_21
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

249-254

Subventions

Organisme : Austrian Science Fund FWF
ID : I 3734
Pays : Austria

Références

Lindley LE, Stojadinovic O, Pastar I et al (2016) Biology and biomarkers for wound healing. Plast Reconstr Surg 138:18S–28S
doi: 10.1097/PRS.0000000000002682
Nasir NAM, Paus R, Ansell DM (2019) Fluorescent cell tracer dye permits real-time assessment of re-epithelialization in a serum-free ex vivo human skin wound assay. Wound Repair Regen 27:126–133
doi: 10.1111/wrr.12688
Ud-Din S, Bayat A (2017) Non-animal models of wound healing in cutaneous repair: in silico, in vitro, ex vivo, and in vivo models of wounds and scars in human skin. Wound Repair Regen 25:164–176
doi: 10.1111/wrr.12513
Zhang GY, Langan EA, Meier NT et al (2019) Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo. PLoS One 14:e0212659
doi: 10.1371/journal.pone.0212659
Stone R, Wall JT, Natesan S (2018) PEG-plasma hydrogels increase epithelialization using a human ex vivo skin model. Int J Mol Sci 19:3156
doi: 10.3390/ijms19103156
Liao T, Lehmann J, Sternstein S et al (2019) Nestin+ progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo. Arch Dermatol Res 311:325–330
doi: 10.1007/s00403-019-01889-x
Krzyszczyk P, Schloss R, Palmer A et al (2018) The role of macrophages in acute and chronic wound healing and interventions to promote pro-wound healing phenotypes. Front Physiol 9:419. https://doi.org/10.3389/fphys.2018.00419
doi: 10.3389/fphys.2018.00419 pubmed: 29765329 pmcid: 5938667
Meier NT, Haslam IS, Pattwell DM (2013) Thyrotropin-releasing hormone (TRH) promotes wound re-epithelialisation in frog and human skin. PLoS One 8(9):e73596
doi: 10.1371/journal.pone.0073596
Krishnaswamy VR, Mintz D, Sagi I (2017) Matrix metalloproteinases: the sculptors of chronic cutaneous wounds. Biochim Biophys Acta 1864:2220–2227
doi: 10.1016/j.bbamcr.2017.08.003
Robins SP, Milne G, Duncan A et al (2003) Increased skin collagen extractability and proportions of collagen type III are not normalized after 6 months healing of human excisional wounds. J Invest Dermatol 121:67–272
doi: 10.1046/j.1523-1747.2003.12373.x
Baltzis D, Eleftheriadou I, Veves A (2014) Pathogenesis and treatment of impaired wound healing in diabetes mellitus: new insights. Adv Ther 31:817–836
doi: 10.1007/s12325-014-0140-x
Boniakowski AE, Kimball AS, Jacobs BN et al (2017) Macrophage-mediated inflammation in normal and diabetic wound healing. J Immunol 199:17–24
doi: 10.4049/jimmunol.1700223
Lassig AAD, Bechtold JE, Lindgren BR et al (2018) Tobacco exposure and wound healing in head and neck surgical wounds. Laryngoscope 128:618–625
doi: 10.1002/lary.26813
Campbell L, Emmerson E, Davies F et al (2010) Estrogen promotes cutaneous wound healing via estrogen receptor β independent of its antiinflammatory activities. J Exp Med 207:1825–1833
doi: 10.1084/jem.20100500
Chéret J, Lebonvallet N, Buhé V et al (2014) Influence of sensory neuropeptides on human cutaneous wound healing process. J Dermatol Sci 74:193–203
doi: 10.1016/j.jdermsci.2014.02.001

Auteurs

Jennifer Gherardini (J)

Monasterium Laboratories, Skin and Hair Research Solutions, Muenster, Germany.

Max van Lessen (M)

Monasterium Laboratories, Skin and Hair Research Solutions, Muenster, Germany.

Ilaria Piccini (I)

Monasterium Laboratories, Skin and Hair Research Solutions, Muenster, Germany.

Janin Edelkamp (J)

Monasterium Laboratories, Skin and Hair Research Solutions, Muenster, Germany.

Marta Bertolini (M)

Monasterium Laboratories, Skin and Hair Research Solutions, Muenster, Germany. m.bertolini@monasteriumlab.com.

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Classifications MeSH