[Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model].

branching morphogenesis embryo in vitro organ culture mice submandibular gland

Journal

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
ISSN: 2618-0456
Titre abrégé: Hua Xi Kou Qiang Yi Xue Za Zhi
Pays: China
ID NLM: 9422648

Informations de publication

Date de publication:
01 Apr 2020
Historique:
entrez: 22 4 2020
pubmed: 22 4 2020
medline: 23 4 2020
Statut: ppublish

Résumé

Branching morphology is important to ensure that the organ can obtain the efficient functional morphology in a limited volume. The submandibular gland is a crucial model for studying the morphological processes of organ branches. Harvesting the submandibular gland from mouse embryo is also an essential research technique. In this paper, a modified method for obtaining the submandibular glands of mouse embryo was introduced, and the whole process of obtaining and establishing in vitro organ culture was briefly introduced to accurately simulate branch morphogenesis for vivo development and related research. 分支形态的形成对于保证器官可以在有限的体积内获得最有效的功能形态具有重要意义。下颌下腺是研究器官分支形态发生过程的重要模型,在小鼠胚胎中获取下颌下腺组织进行体外器官培养是一种重要的研究方法。本文介绍了一种改良的小鼠胚胎下颌下腺获取方法,并将整个获取及建立体外器官培养流程作以简介,以对其在体内发育过程中的分支形态发生过程进行更好体外的模拟,从而能够更好地开展相关研究。.

Autres résumés

Type: Publisher (chi)
分支形态的形成对于保证器官可以在有限的体积内获得最有效的功能形态具有重要意义。下颌下腺是研究器官分支形态发生过程的重要模型,在小鼠胚胎中获取下颌下腺组织进行体外器官培养是一种重要的研究方法。本文介绍了一种改良的小鼠胚胎下颌下腺获取方法,并将整个获取及建立体外器官培养流程作以简介,以对其在体内发育过程中的分支形态发生过程进行更好体外的模拟,从而能够更好地开展相关研究。.

Identifiants

pubmed: 32314900
doi: 10.7518/hxkq.2020.02.020
pmc: PMC7184286
doi:

Types de publication

Journal Article

Langues

chi

Sous-ensembles de citation

IM

Pagination

228-232

Références

Elife. 2017 Mar 21;6:
pubmed: 28323616
Nephrol Nurs J. 2019 Jan-Feb;46(1):43-47
pubmed: 30835095
Biochem Biophys Res Commun. 2018 Sep 26;504(1):263-269
pubmed: 30193734
Elife. 2018 Jul 31;7:
pubmed: 30063208
Differentiation. 2006 Sep;74(7):349-64
pubmed: 16916374
Anat Histol Embryol. 2017 Dec;46(6):600-605
pubmed: 28884513
Dev Growth Differ. 1997 Feb;39(1):1-8
pubmed: 9079029

Auteurs

Zhi-Kai Liu (ZK)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Xiang-He Qiao (XH)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Li-Ming Gou (LM)

Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China.

Chun-Jie Li (CJ)

State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Dept. of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

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Classifications MeSH