An altered heparan sulfate structure in the articular cartilage protects against osteoarthritis.
Aggrecans
/ metabolism
Animals
Anterior Cruciate Ligament
/ surgery
Blotting, Western
Cartilage, Articular
/ metabolism
Chondrocytes
/ metabolism
Disease Models, Animal
Disease Progression
Heparitin Sulfate
/ metabolism
Loss of Function Mutation
Matrix Metalloproteinase 2
/ metabolism
Mice
Mice, Transgenic
N-Acetylglucosaminyltransferases
/ genetics
Osteoarthritis
/ genetics
Real-Time Polymerase Chain Reaction
Sulfotransferases
/ genetics
Aggrecan degradation
Articular cartilage
Ext1
Heparan sulfate
Mmp2
Ndst1
Osteoarthritis
Journal
Osteoarthritis and cartilage
ISSN: 1522-9653
Titre abrégé: Osteoarthritis Cartilage
Pays: England
ID NLM: 9305697
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
23
01
2020
revised:
05
04
2020
accepted:
09
04
2020
pubmed:
22
4
2020
medline:
31
7
2021
entrez:
22
4
2020
Statut:
ppublish
Résumé
Osteoarthritis (OA) is a progressive degenerative disease of the articular cartilage caused by an unbalanced activity of proteases, cytokines and other secreted proteins. Since heparan sulfate (HS) determines the activity of many extracellular factors, we investigated its role in OA progression. To analyze the role of the HS level, OA was induced by anterior cruciate ligament transection (ACLT) in transgenic mice carrying a loss-of-function allele of Ext1 in clones of chondrocytes (Col2-rtTA-Cre;Ext1 All investigated mouse strains showed reduced OA scores (Col2-rtTA-Cre;Ext1 A decreased HS content or a reduced sulfation level protect against OA progression by regulating protease activity rather than expression.
Identifiants
pubmed: 32315715
pii: S1063-4584(20)30980-8
doi: 10.1016/j.joca.2020.04.002
pmc: PMC8422443
mid: NIHMS1736972
pii:
doi:
Substances chimiques
Aggrecans
0
Heparitin Sulfate
9050-30-0
N-Acetylglucosaminyltransferases
EC 2.4.1.-
exostosin-1
EC 2.4.1.224
Sulfotransferases
EC 2.8.2.-
heparitin sulfotransferase
EC 2.8.2.8
Matrix Metalloproteinase 2
EC 3.4.24.24
Mmp2 protein, mouse
EC 3.4.24.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
977-987Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM033063
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare no conflicts of interest.
Références
Matrix Biol. 2020 Nov;93:43-59
pubmed: 32201365
Cold Spring Harb Perspect Biol. 2009 Sep;1(3):a002493
pubmed: 20066107
Dev Biol. 2000 Aug 15;224(2):299-311
pubmed: 10926768
Nature. 1994 Jul 7;370(6484):61-5
pubmed: 8015608
Ann Rheum Dis. 1973 Jul;32(4):387-8
pubmed: 4726075
Arthritis Rheum. 1992 Jul;35(7):806-11
pubmed: 1622419
Histol Histopathol. 2017 Jul;32(7):725-733
pubmed: 27808352
J Clin Invest. 1996 Nov 15;98(10):2292-9
pubmed: 8941646
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2054-9
pubmed: 20080592
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S17-23
pubmed: 20864019
Nat Protoc. 2011 Mar;6(3):388-404
pubmed: 21372818
Biochim Biophys Acta. 2010 Jan;1803(1):55-71
pubmed: 20080133
J Biol Chem. 2010 Jun 18;285(25):19227-34
pubmed: 20404326
Dev Biol. 2013 May 1;377(1):100-12
pubmed: 23458899
Genet Mol Res. 2015 Nov 23;14(4):14811-22
pubmed: 26600542
Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10202-7
pubmed: 20479257
Osteoarthritis Cartilage. 2005 Jul;13(7):632-41
pubmed: 15896985
Bone. 2013 Nov;57(1):220-31
pubmed: 23958822
J Biol Chem. 1995 Feb 10;270(6):2550-6
pubmed: 7852317
Cancer Res. 1995 Jun 15;55(12):2548-55
pubmed: 7780967
Development. 2005 Nov;132(22):5055-68
pubmed: 16236767
J Cell Biol. 2019 Jun 3;218(6):1853-1870
pubmed: 31085560
Dev Dyn. 2007 Feb;236(2):556-63
pubmed: 17183530
Biochim Biophys Acta. 2012 Jan;1824(1):133-45
pubmed: 21777704
Bone. 2018 Jun;111:71-81
pubmed: 29545125
J Theor Biol. 1966 Nov;12(2):276-90
pubmed: 5972197
Am J Pathol. 2019 Mar;189(3):632-647
pubmed: 30553836
Am J Pathol. 2001 Nov;159(5):1777-83
pubmed: 11696438
Genesis. 2002 Jun;33(2):77-80
pubmed: 12112875
Arthritis Rheumatol. 2016 Apr;68(4):868-79
pubmed: 26636652
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13075-80
pubmed: 18725627
J Thromb Haemost. 2008 Jul;6(7):1198-206
pubmed: 18433461
Nat Genet. 1995 Oct;11(2):137-43
pubmed: 7550340
Nature. 2007 Apr 26;446(7139):1030-7
pubmed: 17460664
J Biol Chem. 1992 Feb 25;267(6):3581-4
pubmed: 1371271
Matrix Biol. 2015 May-Jul;44-46:255-63
pubmed: 25701651
Development. 2005 Aug;132(16):3777-86
pubmed: 16020517
Ann Rheum Dis. 1983 Feb;42(1):82-8
pubmed: 6830328
Nat Genet. 1999 Jan;21(1):70-1
pubmed: 9916792
Best Pract Res Clin Rheumatol. 2001 Dec;15(5):805-29
pubmed: 11812023
J Cell Biol. 2003 Jul 7;162(1):139-48
pubmed: 12835312
Am J Pathol. 2015 Jun;185(6):1676-85
pubmed: 25863260
Methods Enzymol. 1995;248:510-28
pubmed: 7674942
Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7):
pubmed: 21690215
Sci Rep. 2019 Mar 20;9(1):4905
pubmed: 30894640
Anal Biochem. 1996 May 1;236(2):353-6
pubmed: 8660517
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
J Clin Invest. 1995 May;95(5):2178-86
pubmed: 7537757
Matrix Biol. 2013 Apr 24;32(3-4):178-87
pubmed: 23339896
Nat Med. 2009 Sep;15(9):1072-6
pubmed: 19684582