Unravelling the consequences of the bacteriophages in human samples.
Ascitic Fluid
/ microbiology
Bacteria
/ classification
Blood Culture
/ methods
Capsid
/ chemistry
Cerebrospinal Fluid
/ microbiology
Filtration
/ methods
Humans
Inoviridae
/ classification
Lysogeny
/ physiology
Molecular Typing
/ methods
Myoviridae
/ classification
Podoviridae
/ classification
RNA, Ribosomal, 16S
/ genetics
Serum
/ microbiology
Siphoviridae
/ classification
Urine
/ microbiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 04 2020
21 04 2020
Historique:
received:
21
11
2019
accepted:
31
03
2020
entrez:
23
4
2020
pubmed:
23
4
2020
medline:
1
12
2020
Statut:
epublish
Résumé
Bacteriophages are abundant in human biomes and therefore in human clinical samples. Although this is usually not considered, they might interfere with the recovery of bacterial pathogens at two levels: 1) by propagating in the enrichment cultures used to isolate the infectious agent, causing the lysis of the bacterial host and 2) by the detection of bacterial genes inside the phage capsids that mislead the presence of the bacterial pathogen. To unravel these interferences, human samples (n = 271) were analyzed and infectious phages were observed in 11% of blood culture, 28% of serum, 45% of ascitic fluid, 14% of cerebrospinal fluid and 23% of urine samples. The genetic content of phage particles from a pool of urine and ascitic fluid samples corresponded to bacteriophages infecting different bacterial genera. In addition, many bacterial genes packaged in the phage capsids, including antibiotic resistance genes and 16S rRNA genes, were detected in the viromes. Phage interference can be minimized applying a simple procedure that reduced the content of phages up to 3 logs while maintaining the bacterial load. This method reduced the detection of phage genes avoiding the interference with molecular detection of bacteria and reduced the phage propagation in the cultures, enhancing the recovery of bacteria up to 6 logs.
Identifiants
pubmed: 32317653
doi: 10.1038/s41598-020-63432-7
pii: 10.1038/s41598-020-63432-7
pmc: PMC7174282
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6737Références
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