EndOxy: Mid-term stability and shear stress resistance of endothelial cells on PDMS gas exchange membranes.
Bioreactors
Cell Adhesion
Dimethylpolysiloxanes
/ chemistry
Extracorporeal Membrane Oxygenation
/ adverse effects
Feasibility Studies
Human Umbilical Vein Endothelial Cells
/ metabolism
Humans
Lab-On-A-Chip Devices
Oligopeptides
/ chemistry
Oxygen
/ metabolism
Oxygenators, Membrane
Stress, Mechanical
biohybrid lung
extracorporeal membrane oxygenation
human umbilical vein endothelial cells
polydimethylsiloxane
tissue engineering
Journal
Artificial organs
ISSN: 1525-1594
Titre abrégé: Artif Organs
Pays: United States
ID NLM: 7802778
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
02
12
2019
revised:
10
03
2020
accepted:
16
04
2020
pubmed:
23
4
2020
medline:
12
10
2021
entrez:
23
4
2020
Statut:
ppublish
Résumé
Endothelialized oxygenator devices (EndOxy) with a physiological, nonthrombogenic, and anti-inflammatory surface offer the potential to overcome current shortcomings of conventional extracorporeal membrane oxygenation such as complications like thromboembolism and bleeding that deteriorate adequate long-term hemocompatibility. The approach of endothelialization of gas exchange membranes, and thus the formation of a nonthrombogenic and anti-inflammatory surface, is promising. In this study, we investigated the mid-term shear stress resistance as well as gas transfer rates and cell densities of endothelial cells seeded on RGD-conjugated polydimethylsiloxane (RGD-PDMS) gas exchange membranes under dynamic conditions. Human umbilical vein endothelial cells were seeded on RGD-PDMS and exposed to defined shear stresses in a microfluidic bioreactor. Endothelial cell morphology was assessed by bright field microscopy and immunocytochemistry. Furthermore, gas transfer measurement of blank, RGD-conjugated, and endothelialized PDMS oxygenator membranes was performed. RGD-PDMS gas exchange membranes proved suitable for the dynamic culture of endothelial cells for up to 21 days at a wall shear stress of 2.9 dyn/cm
Substances chimiques
Dimethylpolysiloxanes
0
Oligopeptides
0
baysilon
63148-62-9
arginyl-glycyl-aspartic acid
78VO7F77PN
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
E419-E433Subventions
Organisme : Aachen Interdisciplinary Center for Clinical Research (IZKF) at RWTH Aachen University Hospital
ID : T12
Organisme : Excellence Initiative of the German Federal and State Governments
ID : i3tm Seed Fund
Organisme : Excellence Initiative of the German Federal and State Governments
ID : STEP2 projects
Informations de copyright
© 2020 The Authors. Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.
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