High-throughput gene screen reveals modulators of nuclear shape.
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
15 06 2020
15 06 2020
Historique:
pubmed:
23
4
2020
medline:
4
6
2021
entrez:
23
4
2020
Statut:
ppublish
Résumé
Irregular nuclear shapes characterized by blebs, lobules, micronuclei, or invaginations are hallmarks of many cancers and human pathologies. Despite the correlation between abnormal nuclear shape and human pathologies, the mechanism by which the cancer nucleus becomes misshapen is not fully understood. Motivated by recent evidence that modifying chromatin condensation can change nuclear morphology, we conducted a high-throughput RNAi screen to identify epigenetic regulators that are required to maintain normal nuclear shape in human breast epithelial MCF-10A cells. We silenced 608 genes in parallel using an epigenetics siRNA library and used an unbiased Fourier analysis approach to quantify nuclear contour irregularity from fluorescent images captured on a high-content microscope. Using this quantitative approach, which we validated with confocal microscopy, we significantly expand the list of epigenetic regulators that impact nuclear morphology.
Identifiants
pubmed: 32320319
doi: 10.1091/mbc.E19-09-0520
pmc: PMC7353136
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1392-1402Subventions
Organisme : NCI NIH HHS
ID : R50 CA211487
Pays : United States
Organisme : NIGMS NIH HHS
ID : R00 GM123195
Pays : United States
Organisme : NIGMS NIH HHS
ID : K99 GM123195
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA172310
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA193419
Pays : United States
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