Profiling of Small Ribosomal Subunits Reveals Modes and Regulation of Translation Initiation.
Kozak sequence
RNA
initiation
next-generation sequencing
ribosome profiling
ribosome recruitment
scanning processivity
small ribosomal subunit (SSU)
translation
uORF
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
21 04 2020
21 04 2020
Historique:
received:
08
11
2019
revised:
28
02
2020
accepted:
27
03
2020
entrez:
23
4
2020
pubmed:
23
4
2020
medline:
15
5
2021
Statut:
ppublish
Résumé
Translation initiation is often attributed as the rate-determining step of eukaryotic protein synthesis and key to gene expression control. Despite this centrality, the series of steps involved in this process is poorly understood. Here, we capture the transcriptome-wide occupancy of ribosomes across all stages of translation initiation, enabling us to characterize the transcriptome-wide dynamics of ribosome recruitment to mRNAs, scanning across 5' UTRs and stop codon recognition, in a higher eukaryote. We provide mechanistic evidence for ribosomes attaching to the mRNA by threading the mRNA through the small subunit. Moreover, we identify features that regulate the recruitment and processivity of scanning ribosomes and redefine optimal initiation contexts. Our approach enables deconvoluting translation initiation into separate stages and identifying regulators at each step.
Identifiants
pubmed: 32320657
pii: S2211-1247(20)30434-4
doi: 10.1016/j.celrep.2020.107534
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107534Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.