Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts.
Animals
Bucladesine
/ pharmacology
Cell Culture Techniques
/ methods
Cell Differentiation
/ drug effects
Cell Line
Chimera
Chromosomes, Mammalian
/ genetics
DNA Methylation
/ genetics
Ectoderm
/ cytology
Gene Expression Regulation
/ drug effects
Giant Cells
/ cytology
Macaca fascicularis
Mice
MicroRNAs
/ genetics
Species Specificity
Stem Cells
/ cytology
Trophoblasts
/ cytology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 04 2020
22 04 2020
Historique:
received:
26
09
2019
accepted:
02
04
2020
entrez:
24
4
2020
pubmed:
24
4
2020
medline:
1
12
2020
Statut:
epublish
Résumé
The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates.
Identifiants
pubmed: 32321940
doi: 10.1038/s41598-020-63602-7
pii: 10.1038/s41598-020-63602-7
pmc: PMC7176671
doi:
Substances chimiques
MicroRNAs
0
Bucladesine
63X7MBT2LQ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6827Références
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