Carotenoids and lipid production from
Lipid
R. toruloides
Tea waste hydrolysate
Torularhodin
Torulene
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
11
2019
accepted:
08
04
2020
entrez:
24
4
2020
pubmed:
24
4
2020
medline:
24
4
2020
Statut:
epublish
Résumé
In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The
Sections du résumé
BACKGROUND
BACKGROUND
In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel
RESULTS
RESULTS
RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance.
CONCLUSION
CONCLUSIONS
Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The
Identifiants
pubmed: 32322304
doi: 10.1186/s13068-020-01712-0
pii: 1712
pmc: PMC7161300
doi:
Types de publication
Journal Article
Langues
eng
Pagination
74Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing InterestsThe authors declare that they have no competing interests.
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