Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion.

expression filamentous fungi genetic engineering genome engineering protein secretion

Journal

Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513

Informations de publication

Date de publication:
2020
Historique:
received: 15 01 2020
accepted: 19 03 2020
entrez: 24 4 2020
pubmed: 24 4 2020
medline: 24 4 2020
Statut: epublish

Résumé

Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.

Identifiants

pubmed: 32322579
doi: 10.3389/fbioe.2020.00293
pmc: PMC7156587
doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

293

Informations de copyright

Copyright © 2020 Wang, Zhong and Xiao.

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Auteurs

Qin Wang (Q)

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Chao Zhong (C)

Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
Materials Synthetic Biology Center, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Han Xiao (H)

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Classifications MeSH