Cystathionine gamma-lyase aggravates periodontal damage in traumatic occlusion mouse models.
alveolar bone resorption
cystathionine γ-lyase
osteoclast
periodontal ligament
traumatic occlusion
Journal
Journal of periodontal research
ISSN: 1600-0765
Titre abrégé: J Periodontal Res
Pays: United States
ID NLM: 0055107
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
20
11
2019
revised:
13
02
2020
accepted:
22
03
2020
pubmed:
24
4
2020
medline:
15
12
2020
entrez:
24
4
2020
Statut:
ppublish
Résumé
Though impacts of traumatic occlusion (TO) on periodontal tissues and roles of cystathionine γ-lyase (Cth) gene in the regulation of bone homeostasis have been studied by many, no consensus has been reached so far on whether TO deteriorates the periodontium and precise roles of Cth in occlusal trauma. Therefore, this study aims to investigate the impacts of TO on periodontal tissues and the involvement of Cth gene. Eighty C57BL/6 wild-type (WT) mice and Cth knockout (Cth It was found that decreased alveolar bone height, expanded bone resorption area, and increased width of periodontal ligament (PDL) occurred in TO models, accompanied by an increased number of osteoclasts in a time-dependent manner by micro-CT and histological staining. Osteoclast-related genes including Ctsk, Mmp9, Rank, Trap, and Rankl/Opg were also up-regulated after one week of modeling. The up-regulated expressions of Cth gene and its protein CTH were observed in TO mouse models. After 1, 2, or 3 weeks of modeling, WT mice showed more severe alveolar bone resorption, wider PDL, higher osteoclast count, and higher levels of osteoclast-related genes Ctsk, Rank, and Rankl/Opg than Cth TO causes a reduction in alveolar bone height and PDL morphological disorder with their severity increases in a time-dependent manner. Cth aggravates periodontal damage caused by TO.
Sections du résumé
BACKGROUND AND OBJECTIVE
OBJECTIVE
Though impacts of traumatic occlusion (TO) on periodontal tissues and roles of cystathionine γ-lyase (Cth) gene in the regulation of bone homeostasis have been studied by many, no consensus has been reached so far on whether TO deteriorates the periodontium and precise roles of Cth in occlusal trauma. Therefore, this study aims to investigate the impacts of TO on periodontal tissues and the involvement of Cth gene.
METHODS
METHODS
Eighty C57BL/6 wild-type (WT) mice and Cth knockout (Cth
RESULTS
RESULTS
It was found that decreased alveolar bone height, expanded bone resorption area, and increased width of periodontal ligament (PDL) occurred in TO models, accompanied by an increased number of osteoclasts in a time-dependent manner by micro-CT and histological staining. Osteoclast-related genes including Ctsk, Mmp9, Rank, Trap, and Rankl/Opg were also up-regulated after one week of modeling. The up-regulated expressions of Cth gene and its protein CTH were observed in TO mouse models. After 1, 2, or 3 weeks of modeling, WT mice showed more severe alveolar bone resorption, wider PDL, higher osteoclast count, and higher levels of osteoclast-related genes Ctsk, Rank, and Rankl/Opg than Cth
CONCLUSION
CONCLUSIONS
TO causes a reduction in alveolar bone height and PDL morphological disorder with their severity increases in a time-dependent manner. Cth aggravates periodontal damage caused by TO.
Substances chimiques
Osteoprotegerin
0
RANK Ligand
0
Cystathionine gamma-Lyase
EC 4.4.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
667-675Subventions
Organisme : National Natural Science Foundation of China
ID : 81870791
Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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