Effect of Cell Labeling on the Function of Human Pluripotent Stem Cell-Derived Cardiomyocytes.
Alternative model
Cardiomyocytes
Cell labeling
Direct label
Stem cells
Journal
International journal of stem cells
ISSN: 2005-3606
Titre abrégé: Int J Stem Cells
Pays: Korea (South)
ID NLM: 101497587
Informations de publication
Date de publication:
30 Jul 2020
30 Jul 2020
Historique:
received:
15
11
2019
revised:
01
03
2020
accepted:
03
03
2020
pubmed:
24
4
2020
medline:
24
4
2020
entrez:
24
4
2020
Statut:
ppublish
Résumé
Cell labeling technologies are required to monitor the fate of transplanted cells in vivo and to select target cells for the observation of certain changes in vitro. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been transplanted for the treatment of heart injuries or used in vitro for preclinical cardiac safety assessments. Cardiomyocyte (CM) labeling has been used in these processes to facilitate target cell monitoring. However, the functional effect of the labeling agent on hiPSC-CMs has not been studied. Therefore, we investigated the effects of labeling agents on CM cellular functions. 3'-Dioctadecyloxacarbocyanine perchlorate (DiO), quantum dots (QDs), and a DNA plasmid expressing EGFP using Lipo2K were used to label hiPSC-CMs. We conclude that the hiPSC-CM labeling with DiO and QDs does not induce arrhythmogenic effects but rather improves the mRNA expression of cardiac ion channels and Ca
Identifiants
pubmed: 32323512
pii: ijsc19138
doi: 10.15283/ijsc19138
pmc: PMC7378900
doi:
Types de publication
Journal Article
Langues
eng
Pagination
287-294Références
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