β-Mangostin inhibits the metastatic power of cervical cancer cells attributing to suppression of JNK2/AP-1/Snail cascade.


Journal

Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222

Informations de publication

Date de publication:
11 2020
Historique:
received: 05 09 2019
revised: 18 03 2020
accepted: 22 03 2020
pubmed: 24 4 2020
medline: 17 3 2021
entrez: 24 4 2020
Statut: ppublish

Résumé

β-Mangostin is a natural mangostin with potent anticancer activity against various cancers. In this study, we further explored the anticancer activity of β-mangostin on cervical cancer cells. β-Mangostin did not affect cell viability and cell cycle distribution in HeLa and SiHa cells; however, it dose-dependently inhibited the migration and invasion of both the human cervical cancer cell lines. In addition, we observed that β-mangostin suppressed the expression of integrin αV and β3 and the downstream focal adhesion kinase/Src signaling. We also found that Snail was involved in the β-mangostin inhibited cell migration and invasion of HeLa cell. Then, our findings showed that β-mangostin reduced both nuclear translocation and messenger RNA expression of AP-1 and demonstrated that AP-1 could target to the Snail promoter and induce Snail expression. Kinase cascade analysis and reporter assay showed that JNK2 was involved in the inhibition of AP-1/Snail axis by β-mangostin in HeLa cells. These results indicate that β-mangostin can inhibit the mobility and invasiveness of cervical cancer cells, which may attribute to the suppression of both integrin/Src signaling and JNK2-mediated AP-1/Snail axis. This suggests that β-mangostin has potential antimetastatic potential against cervical cancer cells.

Identifiants

pubmed: 32324277
doi: 10.1002/jcp.29688
doi:

Substances chimiques

Transcription Factor AP-1 0
Xanthones 0
mangostin U6RIV93RU1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

8446-8460

Informations de copyright

© 2020 Wiley Periodicals, Inc.

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Auteurs

Chun-Shiang Lin (CS)

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.

Chia-Liang Lin (CL)

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.

Tsung-Ho Ying (TH)

Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Hui-Ling Chiou (HL)

Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.

Chia-Hung Hung (CH)

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.

Wei-Shan Liao (WS)

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Department of Clinical Pathology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.

Yi-Hsien Hsieh (YH)

Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.
Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Shao-Hsuan Kao (SH)

Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.

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