Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP-in-Microdroplets Approach.
LAMP
Listeria monocytogenes
SERS
glutathione
gold nanoparticles
microdroplets
microfluidics
pathogens
Journal
Materials (Basel, Switzerland)
ISSN: 1996-1944
Titre abrégé: Materials (Basel)
Pays: Switzerland
ID NLM: 101555929
Informations de publication
Date de publication:
20 Apr 2020
20 Apr 2020
Historique:
received:
29
03
2020
revised:
16
04
2020
accepted:
17
04
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
25
4
2020
Statut:
epublish
Résumé
We developed a droplet-based optofluidic system for the detection of foodborne pathogens. Specifically, the loop-mediated isothermal amplification (LAMP) technique was combined with surface-enhanced Raman scattering (SERS), which offers an excellent method for DNA ultradetection. However, the direct SERS detection of DNA compromises the simplicity of data interpretation due to the variability of its SERS fingerprints. Therefore, we designed an indirect SERS detection method using multifunctional gold nanoparticles (AuNPs) based on the formation of pyrophosphate generated during the DNA amplification by LAMP. Towards this goal, we prepared multifunctional AuNPs involving three components with key roles: (1) thiolated poly(ethylene glycol) as stabilizing agent, (2) 1-naphthalenethiol as Raman reporter, and (3) glutathione as a bioinspired chelating agent of magnesium (II) ions. Thus, the variation in the SERS signal of 1-naphthalenethiol was controlled by the aggregation of AuNPs triggered by the complexation of pyrophosphate and glutathione with free magnesium ions. Using this strategy, we detected
Identifiants
pubmed: 32325992
pii: ma13081934
doi: 10.3390/ma13081934
pmc: PMC7215531
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Regional Development Fund
ID : 030782
Organisme : European Regional Development Fund
ID : FEDER-000019
Organisme : European Regional Development Fund
ID : POCI-01-0247-FEDER-033925
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 713640
Organisme : Ramón Areces Foundation
ID : BEVP30A5827
Organisme : Fundação para a Ciência e a Tecnologia
ID : SFRH/BD/148091/2019
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