Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study.
Lactobacillus
alternative to antibiotics
dysbiosis
estrus synchronization
lactic acid bacteria
vaginitis
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
20 Apr 2020
20 Apr 2020
Historique:
received:
30
03
2020
revised:
16
04
2020
accepted:
17
04
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
25
4
2020
Statut:
epublish
Résumé
Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of Lactobacillus used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze the potential use of probiotics to prevent urogenital infections in sheep. A randomized controlled clinical trial was conducted with 21 one-year-old ewes to develop a model of probiotic infusion in vaginal sponges in order to study their influence in ewe's vaginal microbiota, general health status, fertility and prolificity. Synchronization of estrus was based on intravaginal sponges for 14 days. Bacterial communities (Enterobacteriaceae and lactic acid bacteria) were highly fluctuating over time and between animals. The safety of probiotic infusion (mix of Lactobacillus spp. 60% L. crispatus, 20% L. brevis and 20% L. gasseri) in the vagina of healthy ewes was firstly confirmed. Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (p = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively p = 0.097. These results suggest that Lactobacillus spp. infusion in the ewe's vagina does not affect general health status or fertility.
Identifiants
pubmed: 32326046
pii: ani10040719
doi: 10.3390/ani10040719
pmc: PMC7222760
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Universidad Cardenal Herrera-CEU, CEU Universities
ID : Grant IDOC 17-18
Organisme : Grant Grupos Operativos Suprautonómicos Programa Nacional Desarrollo Rural, Ministerio de Agricultura Pesca y Alimentacíon y FEADER
ID : 20180020007158
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Reprod Sci. 2012 Mar;19(3):235-42
pubmed: 22383775
Reprod Domest Anim. 2001 Apr;36(2):73-7
pubmed: 11328559
Sci Rep. 2014 May 30;4:5136
pubmed: 24875844
Theriogenology. 2016 Mar 15;85(5):856-861
pubmed: 26627933
Nat Rev Microbiol. 2018 Jun 24;16:540-550
pubmed: 29937540
Theriogenology. 2016 Mar 1;85(4):625-37
pubmed: 26549120
J Dairy Sci. 2017 Jan;100(1):479-492
pubmed: 27837977
Reprod Domest Anim. 2011 Apr;46(2):205-8
pubmed: 20456668
Front Vet Sci. 2014 Oct 15;1:19
pubmed: 26664918
Vet Microbiol. 2012 Jun 15;157(3-4):355-62
pubmed: 22284907
Lett Appl Microbiol. 2006 Jul;43(1):91-7
pubmed: 16834727
Front Microbiol. 2017 Jun 08;8:1047
pubmed: 28642755
Drugs. 2006;66(9):1253-61
pubmed: 16827601
J Dairy Sci. 2015 Sep;98(9):6018-28
pubmed: 26188583
J Dairy Sci. 2016 Jan;99(1):141-5
pubmed: 26585470
Animals (Basel). 2018 Nov 29;8(12):
pubmed: 30501021
PLoS One. 2019 Jan 10;14(1):e0200974
pubmed: 30629579
Sci Rep. 2018 Feb 28;8(1):3801
pubmed: 29491439
PLoS One. 2012;7(12):e53048
pubmed: 23300859
PLoS One. 2015 Apr 28;10(4):e0124167
pubmed: 25919010
Annu Rev Microbiol. 2012;66:371-89
pubmed: 22746335
Appl Environ Microbiol. 2015 Sep;81(18):6324-32
pubmed: 26150453
Theriogenology. 2012 May;77(8):1549-56
pubmed: 22289216
Theriogenology. 2005 Jun;63(9):2523-34
pubmed: 15910932
Vet Rec. 1988 Nov 19;123(21):554
pubmed: 3206809
J Anim Sci. 2019 Oct 3;97(10):4298-4304
pubmed: 31250893
Anim Reprod Sci. 2012 Feb;130(3-4):173-9
pubmed: 22325928
Reprod Domest Anim. 2015 Apr;50(2):270-274
pubmed: 25604995
PLoS One. 2015 Nov 24;10(11):e0143294
pubmed: 26599789
Front Microbiol. 2016 Mar 31;7:455
pubmed: 27065999
Can Vet J. 2011 Jan;52(1):43-6
pubmed: 21461205