Amyloid Precursor Protein (APP) Controls the Expression of the Transcriptional Activator Neuronal PAS Domain Protein 4 (NPAS4) and Synaptic GABA Release.
CRISPR-Cas9
GABA
NPAS4
amyloid precursor protein
neuronal differentiation
transcriptome
Journal
eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362
Informations de publication
Date de publication:
Historique:
received:
14
08
2019
revised:
13
03
2020
accepted:
17
03
2020
pubmed:
25
4
2020
medline:
22
6
2021
entrez:
25
4
2020
Statut:
epublish
Résumé
The amyloid precursor protein (APP) has been extensively studied as the precursor of the β-amyloid (Aβ) peptide, the major component of the senile plaques found in the brain of Alzheimer's disease (AD) patients. However, the function of APP per se in neuronal physiology remains to be fully elucidated. APP is expressed at high levels in the brain. It resembles a cell adhesion molecule or a membrane receptor, suggesting that its function relies on cell-cell interaction and/or activation of intracellular signaling pathways. In this respect, the APP intracellular domain (AICD) was reported to act as a transcriptional regulator. Here, we used a transcriptome-based approach to identify the genes transcriptionally regulated by APP in the rodent embryonic cortex and on maturation of primary cortical neurons. Surprisingly, the overall transcriptional changes were subtle, but a more detailed analysis pointed to genes clustered in neuronal-activity dependent pathways. In particular, we observed a decreased transcription of neuronal PAS domain protein 4 (NPAS4) in APP-/- neurons. NPAS4 is an inducible transcription factor (ITF) regulated by neuronal depolarization. The downregulation of NPAS4 co-occurs with an increased production of the inhibitory neurotransmitter GABA and a reduced expression of the GABA
Identifiants
pubmed: 32327470
pii: ENEURO.0322-19.2020
doi: 10.1523/ENEURO.0322-19.2020
pmc: PMC7262005
pii:
doi:
Substances chimiques
Amyloid beta-Peptides
0
Amyloid beta-Protein Precursor
0
Basic Helix-Loop-Helix Transcription Factors
0
Npas4 protein, mouse
0
Transcription Factors
0
gamma-Aminobutyric Acid
56-12-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 Opsomer et al.
Références
Nucleic Acids Res. 2015 Apr 20;43(7):e47
pubmed: 25605792
J Neurosci. 2017 Dec 6;37(49):11947-11966
pubmed: 29101243
J Neurochem. 2012 Jan;120 Suppl 1:109-24
pubmed: 22122663
Exp Brain Res. 2012 Apr;217(3-4):325-9
pubmed: 22349563
Cold Spring Harb Perspect Biol. 2011 Jun 01;3(6):
pubmed: 21555405
Science. 2019 Jan 11;363(6423):
pubmed: 30630900
Nature. 2008 Oct 30;455(7217):1198-204
pubmed: 18815592
Bioinformatics. 2010 Sep 1;26(17):2176-82
pubmed: 20610611
PLoS One. 2008 Aug 21;3(8):e3029
pubmed: 18716656
Front Cell Neurosci. 2018 Sep 14;12:318
pubmed: 30271326
J Neurochem. 2009 Dec;111(6):1501-13
pubmed: 19811606
FEBS Lett. 2013 Jun 27;587(13):2036-45
pubmed: 23707420
J Biol Chem. 2011 Jul 8;286(27):24264-74
pubmed: 21606494
FASEB J. 2016 May;30(5):1696-711
pubmed: 26718890
J Neurosci. 2014 Mar 12;34(11):3826-40
pubmed: 24623762
Cell Death Dis. 2014 Aug 28;5:e1391
pubmed: 25165877
CNS Neurosci Ther. 2012 Apr;18(4):285-94
pubmed: 22070283
Nucleic Acids Res. 2002 Jan 1;30(1):207-10
pubmed: 11752295
J Neurosci. 2015 Dec 9;35(49):16018-33
pubmed: 26658856
Nature. 2013 Nov 7;503(7474):121-5
pubmed: 24201284
Neurol Sci. 2013 Sep;34(9):1575-9
pubmed: 23354600
J Neurosci Methods. 2007 May 15;162(1-2):346-56
pubmed: 17306885
Cell. 1995 May 19;81(4):525-31
pubmed: 7758106
J Cell Biochem. 1997 May;65(2):145-58
pubmed: 9136074
J Neurochem. 2007 Sep;102(6):1928-1940
pubmed: 17540010
PLoS One. 2013 Sep 16;8(9):e74305
pubmed: 24066134
Trends Pharmacol Sci. 2016 May;37(5):390-411
pubmed: 26837733
Physiol Rev. 2009 Oct;89(4):1079-103
pubmed: 19789377
J Neurosci. 2005 Jun 8;25(23):5533-43
pubmed: 15944381
J Neurosci Methods. 2008 Aug 15;173(1):27-33
pubmed: 18585786
Neurobiol Dis. 2008 Aug;31(2):250-60
pubmed: 18585048
Nat Biotechnol. 2014 Sep;32(9):941-6
pubmed: 24952903
Acta Neuropathol Commun. 2014 Mar 31;2:36
pubmed: 24684730
Sci Rep. 2017 Mar 23;7(1):370
pubmed: 28337033
Physiol Rev. 2009 Jan;89(1):121-45
pubmed: 19126756
Nat Rev Neurosci. 2017 May;18(5):281-298
pubmed: 28360418
EMBO Mol Med. 2013 Apr;5(4):608-25
pubmed: 23554170
Neuropharmacology. 1999 Mar;38(3):349-59
pubmed: 10219973
Cold Spring Harb Perspect Med. 2012 Feb;2(2):a006288
pubmed: 22355794
J Neurosci. 1998 Aug 15;18(16):6207-17
pubmed: 9698314
Nat Rev Neurosci. 2002 Sep;3(9):728-39
pubmed: 12209121
Trends Neurosci. 2016 Apr;39(4):264-275
pubmed: 26987258
Cell. 1994 Dec 2;79(5):755-65
pubmed: 8001115
Cell. 2014 May 22;157(5):1216-29
pubmed: 24855953
J Neurosci. 2009 Jun 10;29(23):7459-73
pubmed: 19515914
J Neurosci. 2009 Apr 15;29(15):4708-18
pubmed: 19369541
Neuroscience. 1999 Apr;90(1):1-13
pubmed: 10188929
Prog Neurobiol. 2011 Aug;94(3):223-37
pubmed: 21601615
J Neurochem. 2013 Jan;124(1):123-32
pubmed: 23061396
EMBO J. 2016 Oct 17;35(20):2213-2222
pubmed: 27572463
Transl Psychiatry. 2014 Jun 10;4:e396
pubmed: 26126179
J Neurosci. 2006 Jul 5;26(27):7212-21
pubmed: 16822978
J Neurochem. 2003 Apr;85(2):368-77
pubmed: 12675913
BMC Genomics. 2011 Mar 24;12:160
pubmed: 21435241
Ann Neurol. 2011 May;69(5):819-30
pubmed: 21472769
Neurosci Lett. 2000 Jul 7;288(1):9-12
pubmed: 10869803
Biochem Biophys Res Commun. 2007 Sep 21;361(2):317-22
pubmed: 17651693
PLoS One. 2014 Jun 11;9(6):e99467
pubmed: 24919190
Neuroscience. 2010 Aug 11;169(1):344-56
pubmed: 20451588
J Biol Chem. 2013 Jun 28;288(26):18853-62
pubmed: 23671283
J Neurosci. 2007 Jul 18;27(29):7817-26
pubmed: 17634375
Brain Res. 2015 Sep 24;1621:38-50
pubmed: 25452022
J Neurosci. 2014 Oct 1;34(40):13314-25
pubmed: 25274811
Elife. 2017 Jan 05;6:
pubmed: 28054918
Exp Brain Res. 2012 Apr;217(3-4):441-7
pubmed: 22076403
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Mol Cell Neurosci. 2012 Aug;51(1-2):43-52
pubmed: 22884903
J Neurosci. 2007 Dec 26;27(52):14459-69
pubmed: 18160654
J Biol Chem. 2009 Mar 27;284(13):8495-506
pubmed: 19164281
Cell Res. 2012 Jan;22(1):78-89
pubmed: 21769132
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Brain Res. 1998 Jul 13;799(1):148-55
pubmed: 9666109
J Biol Chem. 2013 Sep 6;288(36):26039-51
pubmed: 23897820
J Biol Chem. 2010 Dec 31;285(53):41443-54
pubmed: 20961856