Differential Effects of the Hormonal and Copper Intrauterine Device on the Endometrial Transcriptome.
Adult
Biopsy
Contraceptive Agents, Female
/ pharmacology
Endometrium
/ metabolism
Female
Gene Expression Profiling
Gene Expression Regulation
/ drug effects
Humans
Intrauterine Devices, Copper
Intrauterine Devices, Medicated
Levonorgestrel
/ pharmacology
Principal Component Analysis
Signal Transduction
/ drug effects
Transcriptome
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 04 2020
23 04 2020
Historique:
received:
13
12
2019
accepted:
02
04
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
1
12
2020
Statut:
epublish
Résumé
The contraceptive effectiveness of intrauterine devices (IUDs) has been attributed in part to a foreign body reaction in the endometrium. We performed this study to better understand mechanisms of action of contraceptives of by studying their effects on endometrial and cervical transcriptomes. We collected endometrial and cervical biopsies from women using the levonorgestrel-releasing intrauterine system (LNG-IUS, n = 11), copper intrauterine device (cu-IUD, n = 13) or levonorgestrel-containing combined oral contraceptives (COC, n = 12), and from women not using contraceptives (control group, n = 11). Transcriptional profiling was performed with Affymetrix arrays, Principal Component Analysis and the bioconductor package limma. In endometrial samples from cu-IUD users, there were no genes with statistically significant differential expression compared to controls. In LNG-IUS users, 2509 genes were differentially expressed and mapped predominantly onto immune and inflammatory pathways. The cervical samples showed no statistically significant differential gene expression compared to controls. Hormonal and copper IUDs have significantly different effects on the endometrial transcriptome, with the LNG-IUS transcriptome showing pronounced inflammation and immune activation compared to controls whereas the cu-IUD transcriptome was indistinguishable from luteal phase endometrium. These findings argue against a foreign body reaction as a common mechanism of action of IUDs.
Identifiants
pubmed: 32327684
doi: 10.1038/s41598-020-63798-8
pii: 10.1038/s41598-020-63798-8
pmc: PMC7181869
doi:
Substances chimiques
Contraceptive Agents, Female
0
Levonorgestrel
5W7SIA7YZW
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6888Subventions
Organisme : NICHD NIH HHS
ID : K12 HD001259
Pays : United States
Organisme : NICHD NIH HHS
ID : K12 HD001262
Pays : United States
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