A Placenta Derived C-Terminal Fragment of β-Hemoglobin With Combined Antibacterial and Antiviral Activity.
AMP
antiviral
hemoglobin fragment
placenta
proteolytic generation
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
14
11
2019
accepted:
09
03
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
25
4
2020
Statut:
epublish
Résumé
The placenta acts as physical and immunological barrier against the transmission of viruses and bacteria from mother to fetus. However, the specific mechanisms by which the placenta protects the developing fetus from viral and bacterial pathogens are poorly understood. To identify placental peptides and small proteins protecting from viral and bacterial infections, we generated a peptide library from 10 kg placenta by chromatographic means. Screening the resulting 250 fractions against Herpes-Simplex-Virus 2 (HSV-2), which is rarely transmitted through the placenta, in a cell-based system identified two adjacent fractions with significant antiviral activity. Further rounds of chromatographic purification and anti-HSV-2 testing allowed to purify the bioactive peptide. Mass spectrometry revealed the presence of a 36-mer derived from the C-terminal region of the hemoglobin β subunit. The purified and corresponding chemically synthesized peptide, termed HBB(112-147), inhibited HSV-2 infection in a dose-dependent manner, with a mean IC
Identifiants
pubmed: 32328038
doi: 10.3389/fmicb.2020.00508
pmc: PMC7153485
doi:
Types de publication
Journal Article
Langues
eng
Pagination
508Informations de copyright
Copyright © 2020 Groß, Bauer, Krüger, Rücker-Braun, Olari, Ständker, Preising, Rodríguez, Conzelmann, Gerbl, Sauter, Kirchhoff, Hagemann, Gačanin, Weil, Ruiz-Blanco, Sanchez-Garcia, Forssmann, Mankertz, Santibanez, Stenger, Walther, Wiese, Spellerberg and Münch.
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