Vitamin A prevents lipopolysaccharide-induced injury on tight junctions in mice.
inflammation
intestine
tight junctions
vitamin A
Journal
Food science & nutrition
ISSN: 2048-7177
Titre abrégé: Food Sci Nutr
Pays: United States
ID NLM: 101605473
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
31
08
2019
revised:
14
12
2019
accepted:
21
12
2019
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
25
4
2020
Statut:
epublish
Résumé
Vitamin A (VA) is one of the most widely used food supplements, but its molecular mechanisms largely remain elusive. Previously, we have demonstrated that VA inhibits the action of lipopolysaccharide (LPS) on intestinal epithelial barrier function and tight junction proteins using IPEC-J2 cells, one of representative intestinal cell lines as a cellular model. These exciting findings stimulated us continue to determine the effects of VA on LPS-induced damage of intestinal integrity in mice. Our results demonstrated that LPS treatment caused reductions of the mRNA levels of tight junction proteins including Zo-1, Occludin, and Claudin-1, well-known biomarkers of intestinal integrity, and these reductions were reversed by VA pretreatment. Intestinal immunofluorescent results of Claudin-1 revealed that LPS disrupted the structure of tight junction and reduced the expression of Claudin-1 at protein level, which was reversed by VA pretreatment. These results suggest that VA may exert a profound role on preventing intestinal inflammation in vivo.
Identifiants
pubmed: 32328260
doi: 10.1002/fsn3.1481
pii: FSN31481
pmc: PMC7174240
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1942-1948Informations de copyright
© 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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