A direct role for SNX9 in the biogenesis of filopodia.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 04 2020
06 04 2020
Historique:
received:
08
10
2019
revised:
24
01
2020
accepted:
31
01
2020
entrez:
25
4
2020
pubmed:
25
4
2020
medline:
29
12
2020
Statut:
ppublish
Résumé
Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.
Identifiants
pubmed: 32328641
pii: 151579
doi: 10.1083/jcb.201909178
pmc: PMC7147113
pii:
doi:
Substances chimiques
SNX9 protein, Xenopus
0
Sorting Nexins
0
Xenopus Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 281971
Pays : International
Organisme : Cancer Research UK
ID : C6946/A14492
Pays : United Kingdom
Organisme : Medical Research Council
ID : N000846/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 105602/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/T030089/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108467/Z/15/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 092096
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT095829AIA
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Jarsch et al.
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