Isolation and characterization of new Puumala orthohantavirus strains from Germany.
Bank vole
Cell culture
Glycoprotein-specific antibodies
Puumala orthohantavirus
Virus adaptation
Journal
Virus genes
ISSN: 1572-994X
Titre abrégé: Virus Genes
Pays: United States
ID NLM: 8803967
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
20
11
2019
accepted:
03
04
2020
pubmed:
25
4
2020
medline:
28
1
2021
entrez:
25
4
2020
Statut:
ppublish
Résumé
Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city Osnabrück, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies.
Identifiants
pubmed: 32328924
doi: 10.1007/s11262-020-01755-3
pii: 10.1007/s11262-020-01755-3
pmc: PMC7329759
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
448-460Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 01KI1721A
Organisme : Bundesministerium für Bildung und Forschung
ID : FKZ 01KI1730
Organisme : Bundesministerium für Bildung und Forschung
ID : 03ZZ0821A
Organisme : Bundesministerium für Bildung und Forschung
ID : 01KI1721H
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