Therapeutic effects of extracellular vesicles from human adipose-derived mesenchymal stem cells on chronic experimental autoimmune encephalomyelitis.
Adipose Tissue
/ metabolism
Animals
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental
/ drug therapy
Extracellular Vesicles
/ metabolism
Humans
Inflammation
/ metabolism
Mesenchymal Stem Cell Transplantation
/ methods
Mesenchymal Stem Cells
/ metabolism
Mice, Inbred C57BL
Spinal Cord
/ metabolism
T-Lymphocytes, Regulatory
/ immunology
adipose tissue
experimental autoimmune encephalomyelitis
extracellular vesicles
human adipose-derive mesenchymal stem cells
mesenchymal stem cells
multiple sclerosis
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
21
02
2020
revised:
05
04
2020
accepted:
06
04
2020
pubmed:
25
4
2020
medline:
17
3
2021
entrez:
25
4
2020
Statut:
ppublish
Résumé
Since in cell therapy, there are always concerns about immune rejection, genetic disability, and malignancies, special attention has been paid to extracellular vesicles (EVs) which are secreted by mesenchymal stem cells (MSCs). In the present study, we assessed and compared the therapeutic effects of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-EVs from adipose tissue on experimental autoimmune encephalomyelitis (EAE). After induction of EAE in C57Bl/6 mice, they were treated with hADSCs, hADSC-EVs, or vehicle intravenously. The clinical score of all mice was recorded every other day. Mice were killed at Day 30 and splenocytes were isolated for proliferation assay and determination of the frequency of Treg cells by flow cytometry. Leukocyte infiltration by hematoxylin and eosin, percentages of demyelination areas by luxol fast blue, and mean fluorescence intensity of oligodendrocyte transcription factor 2 (OLIG2) and myelin basic protein (MBP) by immunohistochemistry were assessed in the spinal cord. Our results showed that the maximum mean clinical score and myelin oligodendrocyte glycoprotein-induced proliferation of splenocytes in hADSC- and hADSC-EV-treated mice were significantly lower than the control mice (p < .05). We also demonstrated that the frequency of CD4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8779-8790Informations de copyright
© 2020 Wiley Periodicals, Inc.
Références
Anderson, P., Gonzalez-Rey, E., O'Valle, F., Martin, F., Oliver, F. J., & Delgado, M. (2017). Allogeneic adipose-derived mesenchymal stromal cells ameliorate experimental autoimmune encephalomyelitis by regulating self-reactive T cell responses and dendritic cell function. Stem Cells International, 2017, 1-15.
Bai, L., Shao, H., Wang, H., Zhang, Z., Su, C., Dong, L., … Zhang, X. (2017). Effects of mesenchymal stem cell-derived exosomes on experimental autoimmune uveitis. Scientific Reports, 7(1), 4323.
Bi, B., Schmitt, R., Israilova, M., Nishio, H., & Cantley, L. G. (2007). Stromal cells protect against acute tubular injury via an endocrine effect. Journal of the American Society of Nephrology, 18(9), 2486-2496.
Blazquez, R., Sanchez-Margallo, F. M., de la Rosa, O., Dalemans, W., Álvarez, V., Tarazona, R., & Casado, J. G. (2014). Immunomodulatory potential of human adipose mesenchymal stem cells derived exosomes on in vitro stimulated T cells. Frontiers in Immunology, 5, 556.
Brownlee, W. J., Hardy, T. A., Fazekas, F., & Miller, D. H. (2017). Diagnosis of multiple sclerosis: Progress and challenges. The Lancet, 389(10076), 1336-1346.
Chopp, M., & Zhang, Z. G. (2015). Emerging potential of exosomes and noncoding microRNAs for the treatment of neurological injury/diseases . Expert Opinion on Emerging Drugs, 20(4), 523-526.
Cohen, J. A., Imrey, P. B., Planchon, S. M., Bermel, R. A., Fisher, E., Fox, R. J., … Lazarus, H. M. (2018). Pilot trial of intravenous autologous culture-expanded mesenchymal stem cell transplantation in multiple sclerosis. Multiple Sclerosis Journal, 24(4), 501-511.
Conforti, A., Scarsella, M., Starc, N., Giorda, E., Biagini, S., Proia, A., … Bernardo, M. E. (2014). Microvescicles derived from mesenchymal stromal cells are not as effective as their cellular counterpart in the ability to modulate immune responses in vitro. Stem Cells and Development, 23(21), 2591-2599.
Constantin, G., Marconi, S., Rossi, B., Angiari, S., Calderan, L., Anghileri, E., … Bonetti, B. (2009). Adipose-derived mesenchymal stem cells ameliorate chronic experimental autoimmune encephalomyelitis. Stem Cells, 27(10), 2624-2635.
Del Fattore, A., Luciano, R., Pascucci, L., Goffredo, B. M., Giorda, E., Scapaticci, M., … Muraca, M. (2015). Immunoregulatory effects of mesenchymal stem cell-derived extracellular vesicles on T lymphocytes. Cell Transplantation, 24(12), 2615-2627.
Drommelschmidt, K., Serdar, M., Bendix, I., Herz, J., Bertling, F., Prager, S., … Felderhoff-Müser, U. (2017). Mesenchymal stem cell-derived extracellular vesicles ameliorate inflammation-induced preterm brain injury. Brain, Behavior, and Immunity, 60, 220-232.
Farinazzo, A., Angiari, S., Turano, E., Bistaffa, E., Dusi, S., Ruggieri, S., & Bonetti, B. (2018). Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis. Scientific Reports, 8(1), 7473.
Frese, L., Dijkman, P. E., & Hoerstrup, S. P. (2016). Adipose tissue-derived stem cells in regenerative medicine. Transfusion Medicine and Hemotherapy, 43(4), 268-274.
Gerdoni, E., Gallo, B., Casazza, S., Musio, S., Bonanni, I., Pedemonte, E., … Uccelli, A. (2007). Mesenchymal stem cells effectively modulate pathogenic immune response in experimental autoimmune encephalomyelitis. Annals of Neurology, 61(3), 219-227.
Gouveia de Andrade, A. V., Bertolino, G., Riewaldt, J., Bieback, K., Karbanová, J., Odendahl, M., … Tonn, T. (2015). Extracellular vesicles secreted by bone marrow-and adipose tissue-derived mesenchymal stromal cells fail to suppress lymphocyte proliferation. Stem Cells and Development, 24(11), 1374-1376.
Haghmorad, D., Mahmoudi, M. B., Salehipour, Z., Jalayer, Z., Momtazi Brojeni, A. A., Rastin, M., … Mahmoudi, M. (2017). Hesperidin ameliorates immunological outcome and reduces neuroinflammation in the mouse model of multiple sclerosis. Journal of Neuroimmunology, 302, 23-33.
Harris, V. K., Vyshkina, T., & Sadiq, S. A. (2016). Clinical safety of intrathecal administration of mesenchymal stromal cell-derived neural progenitors in multiple sclerosis. Cytotherapy, 18(12), 1476-1482.
Jafarinia, M., Alsahebfosoul, F., Salehi, H., Eskandari, N., & Ganjalikhani-Hakemi, M. (2020). Mesenchymal Stem Cell-Derived Extracellular Vesicles: A Novel Cell-Free Therapy. Immunological Investigations, 1-23.
Kalra, H., Simpson, R. J., Ji, H., Aikawa, E., Altevogt, P., Askenase, P., … Mathivanan, S. (2012). Vesiclepedia: A compendium for extracellular vesicles with continuous community annotation. PLoS Biology, 10(12):e1001450.
Kassis, I., Grigoriadis, N., Gowda-Kurkalli, B., Mizrachi-Kol, R., Ben-Hur, T., Slavin, S., … Karussis, D. (2008). Neuroprotection and immunomodulation with mesenchymal stem cells in chronic experimental autoimmune encephalomyelitis. Archives of Neurology, 65(6), 753-761.
Kassis, I., Petrou, P., Halimi, M., & Karussis, D. (2013). Mesenchymal stem cells (MSC) derived from mice with experimental autoimmune encephalomyelitis (EAE) suppress EAE and have similar biological properties with MSC from healthy donors. Immunology Letters, 154(1-2), 70-76.
Katsha, A. M., Ohkouchi, S., Xin, H., Kanehira, M., Sun, R., Nukiwa, T., & Saijo, Y. (2011). Paracrine factors of multipotent stromal cells ameliorate lung injury in an elastase-induced emphysema model. Molecular Therapy, 19(1), 196-203.
Kordelas, L., Rebmann, V., Ludwig, A., Radtke, S., Ruesing, J., Doeppner, T., … Giebel, B. (2014). MSC-derived exosomes: A novel tool to treat therapy-refractory graft-versus-host disease. Leukemia, 28(4), 970-973.
Lai, R. C., Arslan, F., Lee, M. M., Sze, N. S. K., Choo, A., Chen, T. S., … Lim, S. K. (2010). Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Research, 4(3), 214-222.
Laso-García, F., Ramos-Cejudo, J., Carrillo-Salinas, F. J., Otero-Ortega, L., Feliú, A., Gómez-de Frutos, M., … Gutiérrez-Fernández, M. (2018). Therapeutic potential of extracellular vesicles derived from human mesenchymal stem cells in a model of progressive multiple sclerosis. PLoS One, 13(9):e0202590.
Li, J., Chen, Y., Chen, Z., Huang, Y., Yang, D., Su, Z., … Zhang, X. (2017). Therapeutic effects of human adipose tissue-derived stem cell (hADSC) transplantation on experimental autoimmune encephalomyelitis (EAE) mice. Scientific Reports, 7, 42695.
Lorscheider, J., Jokubaitis, V. G., Spelman, T., Izquierdo, G., Lugaresi, A., Havrdova, E., … Kalincik, T. SBase Study Group (2017). Anti-inflammatory disease-modifying treatment and short-term disability progression in SPMS. Neurology, 89(10), 1050-1059.
Mahmoodi, M., Amiri, H., Ayoobi, F., Rahmani, M., Taghipour, Z., Ghavamabadi, R. T., … Sankian, M. (2019). Carvacrol ameliorates experimental autoimmune encephalomyelitis through modulating pro-and anti-inflammatory cytokines. Life Sciences, 219, 257-263.
Mokarizadeh, A., Delirezh, N., Morshedi, A., Mosayebi, G., Farshid, A. -A., & Mardani, K. (2012). Microvesicles derived from mesenchymal stem cells: Potent organelles for induction of tolerogenic signaling. Immunology Letters, 147(1-2), 47-54.
Nakamura, Y., Miyaki, S., Ishitobi, H., Matsuyama, S., Nakasa, T., Kamei, N., … Ochi, M. (2015). Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration. FEBS Letters, 589(11), 1257-1265.
Nassar, W., El-Ansary, M., Sabry, D., Mostafa, M. A., Fayad, T., Kotb, E., … Adel, H. (2016). Umbilical cord mesenchymal stem cells derived extracellular vesicles can safely ameliorate the progression of chronic kidney diseases. Biomaterials Research, 20(1), 21.
Otero-Ortega, L., Laso-García, F., del Carmen Gómez-de Frutos, M., Rodríguez-Frutos, B., Pascual-Guerra, J., Fuentes, B., … Gutiérrez-Fernández, M. (2017). White matter repair after extracellular vesicles administration in an experimental animal model of subcortical stroke. Scientific Reports, 7, 44433.
Pusic, K. M., Pusic, A. D., & Kraig, R. P. (2016). Environmental enrichment stimulates immune cell secretion of exosomes that promote CNS myelination and may regulate inflammation. Cellular and Molecular Neurobiology, 36(3), 313-325.
Rad, F., Ghorbani, M., Roushandeh, A. M., & Roudkenar, M. H. (2019). Mesenchymal stem cell-based therapy for autoimmune diseases: Emerging roles of extracellular vesicles. Molecular Biology Reports, 46(1), 1533-1549.
Reis, L. A., Borges, F. T., Simoes, M. J., Borges, A. A., Sinigaglia-Coimbra, R., & Schor, N. (2012). Bone marrow-derived mesenchymal stem cells repaired but did not prevent gentamicin-induced acute kidney injury through paracrine effects in rats. PLoS One, 7(9):e44092.
Riazifar, M., Mohammadi, M. R., Pone, E. J., Yeri, A., Lässer, C., Segaliny, A. I., … Zhao, W. (2019). Stem cell-derived exosomes as nanotherapeutics for autoimmune and neurodegenerative disorders. ACS Nano, 13, 6670-6688.
Sarvar, D. P., Shamsasenjan, K., & Akbarzadehlaleh, P. (2016). Mesenchymal stem cell-derived exosomes: New opportunity in cell-free therapy. Advanced Pharmaceutical Bulletin, 6(3), 293-299.
Seo, Y., Kim, H.-S., & Hong, I.-S. (2019). Stem cell-derived extracellular vesicles as immunomodulatory therapeutics. Stem Cells International, 2019, 2019-10.
Stepien, A., Dabrowska, N. L., Maciagowska, M., Macoch, R. P., Zolocinska, A., Mazur, S., … Pojda, Z. (2016). Clinical application of autologous adipose stem cells in patients with multiple sclerosis: Preliminary results. Mediators of Inflammation, 2016, 2016-5.
Tamura, R., Uemoto, S., & Tabata, Y. (2016). Immunosuppressive effect of mesenchymal stem cell-derived exosomes on a concanavalin A-induced liver injury model. Inflammation and Regeneration, 36(1), 26.
Tan, C. Y., Lai, R. C., Wong, W., Dan, Y. Y., Lim, S.-K., & Ho, H. K. (2014). Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models. Stem Cell Research & Therapy, 5(3), 76.
Toma, C., Wagner, W. R., Bowry, S., Schwartz, A., & Villanueva, F. (2009). Fate of culture-expanded mesenchymal stem cells in the microvasculature: In vivo observations of cell kinetics. Circulation Research, 104(3), 398-402.
Wen, D., Peng, Y., Liu, D., Weizmann, Y., & Mahato, R. I. (2016). Mesenchymal stem cell and derived exosome as small RNA carrier and Immunomodulator to improve islet transplantation. Journal of Controlled Release, 238, 166-175.
Wickman, G., Julian, L., & Olson, M. (2012). How apoptotic cells aid in the removal of their own cold dead bodies. Cell Death and Differentiation, 19(5), 735-742.
Yuan, Y., Du, W., Liu, J., Ma, W., Zhang, L., Du, Z., & Cai, B. (2018). Stem cell-derived exosome in cardiovascular diseases: Macro roles of micro particles. Frontiers in Pharmacology, 9, 547.
Zhang, B., Yin, Y., Lai, R. C., Tan, S. S., Choo, A. B. H., & Lim, S. K. (2013). Mesenchymal stem cells secrete immunologically active exosomes. Stem Cells and Development, 23(11), 1233-1244.
Zhang, Y., Chopp, M., Meng, Y., Katakowski, M., Xin, H., Mahmood, A., & Xiong, Y. (2015). Effect of exosomes derived from multipluripotent mesenchymal stromal cells on functional recovery and neurovascular plasticity in rats after traumatic brain injury. Journal of Neurosurgery, 122(4), 856-867.