Chromosome dynamics and spindle microtubule establishment in mouse embryos.
embryo
error correction
mitosis
spindle
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
23
11
2019
revised:
25
03
2020
accepted:
31
03
2020
pubmed:
25
4
2020
medline:
20
1
2021
entrez:
25
4
2020
Statut:
ppublish
Résumé
Chromosome segregation errors in mammalian embryos are common and jeopardize embryo health. Here, we perform for the first time 4-Dimensional imaging and tracking of chromosomes and centromeres through each preimplantation mitotic cell division in mouse embryos to define the normal dynamics of chromosome segregation. We show that a microtubule (MT)-dependent inward movement of chromosomes occurs at the time of nuclear envelope breakdown (NEBD), particularly in the earliest cell divisions, to position chromosomes prior to spindle assembly. Establishment of a rudimentary metaphase plate occurs immediately after NEBD, and is followed by a progressive alignment and biorientation of mitotic chromosomes. Stable end-on kinetochore-MT attachments form rapidly and attachment errors are uncommon. Altogether our data describe a rapid and efficient spindle assembly pathway that apparently minimizes the need for canonical MT attachment error correction in normally dividing embryos.
Identifiants
pubmed: 32329130
doi: 10.1096/fj.201902947R
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8057-8067Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2020 Her Majesty the Queen in Right of Canada The FASEB Journal © 2020 John Wiley & Sons Ltd.
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