Cryogenic Correlative Single-Particle Photoluminescence Spectroscopy and Electron Tomography for Investigation of Nanomaterials.
correlative light electron microscopy (CLEM)
cryogenic electron tomography
fluorescence spectroscopy
nanoparticles
single-molecule studies
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
24
02
2020
pubmed:
25
4
2020
medline:
19
3
2021
entrez:
25
4
2020
Statut:
ppublish
Résumé
Cryogenic single-particle photoluminescence (PL) spectroscopy has been used with great success to directly observe the heterogeneous photophysical states present in a population of luminescent particles. Cryogenic electron tomography provides complementary nanometer scale structural information to PL spectroscopy, but the two techniques have not been correlated due to technical challenges. Here, we present a method for correlating single-particle information from these two powerful microscopy modalities. We simultaneously observe PL brightness, emission spectrum, and in-plane excitation dipole orientation of CdSSe/ZnS quantum dots suspended in vitreous ice. Stable and fluctuating emitters were observed, as well as a surprising splitting of the PL spectrum into two bands with an average energy separation of 80 meV. In some cases, the onset of the splitting corresponded to changes in the in-plane excitation dipole orientation. These dynamics were assigned to structures of individual quantum dots and the excitation dipoles were visualized in the context of structural features.
Identifiants
pubmed: 32330371
doi: 10.1002/anie.202002856
pmc: PMC7894979
mid: NIHMS1622105
doi:
Substances chimiques
Cadmium Compounds
0
Selenium Compounds
0
Sulfides
0
Zinc Compounds
0
cadmium sulfide
057EZR4Z7Q
cadmium selenide
A7F646JC5C
zinc sulfide
KPS085631O
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
15642-15648Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103832
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR026780
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41GM103832
Pays : United States
Informations de copyright
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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