Linc-FOXD3 knockdown enhances hippocampal NSCs activation through upregulation of the Wnt/β-catenin pathway.
Animals
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Forkhead Transcription Factors
/ genetics
Hippocampus
/ drug effects
Male
Mice, Inbred C57BL
Neural Stem Cells
/ drug effects
Neurogenesis
/ drug effects
RNA, Long Noncoding
/ genetics
Repressor Proteins
/ genetics
Up-Regulation
Wnt Proteins
/ metabolism
Wnt Signaling Pathway
/ drug effects
beta Catenin
/ drug effects
Hippocampus
NSCs
lincRNAs
β-catenin
Journal
Neuroscience letters
ISSN: 1872-7972
Titre abrégé: Neurosci Lett
Pays: Ireland
ID NLM: 7600130
Informations de publication
Date de publication:
11 06 2020
11 06 2020
Historique:
received:
03
12
2019
revised:
31
03
2020
accepted:
14
04
2020
pubmed:
25
4
2020
medline:
15
5
2021
entrez:
25
4
2020
Statut:
ppublish
Résumé
Hippocampal neural stem cells (H-NSCs) self-renewal and neurogenesis decrease with aging, but the intrinsic mechanism is unclear. In the current study, we detected the expression level of 8 conserved long intergenic noncoding RNAs (lincRNAs) in H-NSCs during aging, and investigated the function and mechanism of lincRNAs in regulating of H-NSCs. We found the proliferation and neuronal-differentiation capacities of H-NSCs reduced with aging and that this effect was accompanied by an increase in linc-FOXD3. Linc-FOXD3 knockdown improved H-NSCs proliferation and neuronal-differentiation capacities. Further mechanistic studies revealed that the effect of linc-FOXD3 knockdown on H-NSCs phenotypes was partially mediated by the up-regulation of Wnt/β-catenin pathway. Thus, our study provides evidence that linc-FOXD3 could be a promising therapeutic target for the recovery of H-NSCs function during aging.
Identifiants
pubmed: 32330488
pii: S0304-3940(20)30261-5
doi: 10.1016/j.neulet.2020.134991
pii:
doi:
Substances chimiques
Forkhead Transcription Factors
0
Foxd3 protein, mouse
0
RNA, Long Noncoding
0
Repressor Proteins
0
Wnt Proteins
0
beta Catenin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
134991Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interests.