Mass Spectrometry-Based Proteomic Characterization of Cutaneous Melanoma Ectosomes Reveals the Presence of Cancer-Related Molecules.
Biomarkers
Cell Line, Tumor
Cell-Derived Microparticles
/ metabolism
Chromatography, Liquid
Computational Biology
/ methods
Exosomes
/ metabolism
Gene Expression Profiling
Gene Ontology
Humans
Melanoma
/ genetics
Proteomics
Skin Neoplasms
/ genetics
Tandem Mass Spectrometry
Melanoma, Cutaneous Malignant
biomarkers
cutaneous melanoma
ectosomes
extracellular vesicles
invasion
metastasis
proteomics
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Apr 2020
22 Apr 2020
Historique:
received:
23
03
2020
revised:
20
04
2020
accepted:
20
04
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
22
1
2021
Statut:
epublish
Résumé
Cutaneous melanoma (CM) is an aggressive type of skin cancer for which effective biomarkers are still needed. Recently, the protein content of extracellular vesicles (ectosomes and exosomes) became increasingly investigated in terms of its functional role in CM and as a source of novel biomarkers; however, the data concerning the proteome of CM-derived ectosomes is very limited. We used the shotgun nanoLC-MS/MS approach to the profile protein content of ectosomes from primary (WM115, WM793) and metastatic (WM266-4, WM1205Lu) CM cell lines. Additionally, the effect exerted by CM ectosomes on recipient cells was assessed in terms of cell proliferation (Alamar Blue assay) and migratory properties (wound healing assay). All cell lines secreted heterogeneous populations of ectosomes enriched in the common set of proteins. A total of 1507 unique proteins were identified, with many of them involved in cancer cell proliferation, migration, escape from apoptosis, epithelial-mesenchymal transition and angiogenesis. Isolated ectosomes increased proliferation and motility of recipient cells, likely due to the ectosomal transfer of different cancer-promoting molecules. Taken together, these results confirm the significant role of ectosomes in several biological processes leading to CM development and progression, and might be used as a starting point for further studies exploring their diagnostic and prognostic potential.
Identifiants
pubmed: 32331267
pii: ijms21082934
doi: 10.3390/ijms21082934
pmc: PMC7215915
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Narodowe Centrum Nauki
ID : 2013/11/B/NZ4/04315
Organisme : Uniwersytet Jagielloński w Krakowie
ID : K/DSC/005541, N18/DBS/000007
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Life Sci. 2018 Aug 15;207:395-411
pubmed: 29959030
J Extracell Vesicles. 2019 Aug 27;8(1):1635420
pubmed: 31497264
Mol Carcinog. 2014 Aug;53(8):635-47
pubmed: 23625515
Cancer Lett. 2015 May 1;360(2):125-33
pubmed: 25661735
Cancer Med. 2014 Jun;3(3):500-13
pubmed: 24644264
Exp Cell Res. 1995 Jul;219(1):233-42
pubmed: 7628538
Anticancer Res. 2016 Dec;36(12):6449-6456
pubmed: 27919967
J Clin Oncol. 2009 May 1;27(13):2199-208
pubmed: 19307507
Cancer Genomics Proteomics. 2010 Jan-Feb;7(1):17-23
pubmed: 20181627
Anticancer Res. 2014 Dec;34(12):7091-6
pubmed: 25503136
Thromb Haemost. 2011 Oct;106(4):712-23
pubmed: 21800005
Pigment Cell Melanoma Res. 2015 Jul;28(4):464-75
pubmed: 25950383
Am J Pathol. 2000 May;156(5):1515-25
pubmed: 10793063
Anticancer Res. 2015 Dec;35(12):6755-60
pubmed: 26637892
J Proteome Res. 2014 Nov 7;13(11):5031-40
pubmed: 25322343
Proc Natl Acad Sci U S A. 2009 Mar 10;106(10):3794-9
pubmed: 19234131
Int J Mol Sci. 2019 Aug 02;20(15):
pubmed: 31382537
Mol Med. 2012 Dec 06;18:1269-80
pubmed: 22952058
Nat Commun. 2015 Apr 21;6:6919
pubmed: 25897521
Neoplasia. 2007 Apr;9(4):349-57
pubmed: 17460779
Cell Cycle. 2011 Sep 1;10(17):2924-36
pubmed: 21857157
Oncogenesis. 2015 Aug 17;4:e163
pubmed: 26280654
Br J Cancer. 2010 Mar 30;102(7):1157-62
pubmed: 20234362
Acta Pharmacol Sin. 2014 Feb;35(2):230-8
pubmed: 24374814
Oncotarget. 2016 Aug 30;7(35):56279-56294
pubmed: 27462921
J Extracell Vesicles. 2020 Feb 11;9(1):1722433
pubmed: 32128073
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1379-84
pubmed: 21220330
Int J Cancer. 2015 Jan 15;136(2):462-75
pubmed: 24889539
J Cell Biol. 2013 Feb 18;200(4):373-83
pubmed: 23420871
Biochim Biophys Acta. 2009 Jan;1794(1):61-9
pubmed: 18952200
Nat Commun. 2016 Jan 29;7:10582
pubmed: 26822383
Clin Chim Acta. 2016 Feb 15;454:28-32
pubmed: 26724367
Sci Rep. 2015 Aug 25;5:13006
pubmed: 26302712
Bioscience. 2015 Aug 1;65(8):783-797
pubmed: 26955082
Am J Pathol. 1993 Aug;143(2):528-37
pubmed: 8342600
Exp Cell Res. 2004 May 1;295(2):315-29
pubmed: 15093732
J Cell Biol. 1991 Dec;115(5):1427-36
pubmed: 1955483
Tumour Biol. 2015 Dec;36(12):9649-59
pubmed: 26150337
Br J Dermatol. 2017 Jul;177(1):134-140
pubmed: 28369739
Mol Syst Biol. 2014 Oct 30;10:757
pubmed: 25358341
Am J Pathol. 1998 Nov;153(5):1435-42
pubmed: 9811334
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6028-32
pubmed: 2068080
Cancer Res. 2006 Sep 15;66(18):9290-8
pubmed: 16982774
J Proteome Res. 2008 Sep;7(9):4107-18
pubmed: 18698805
Adv Exp Med Biol. 2015;867:115-24
pubmed: 26530363
J Natl Cancer Inst. 1985 Feb;74(2):283-9
pubmed: 3856042
Eur J Surg Oncol. 2012 Apr;38(4):281-5
pubmed: 22240030
Anticancer Res. 2011 Apr;31(4):1329-35
pubmed: 21508383
Cancer Res. 2003 Oct 15;63(20):6716-25
pubmed: 14583466