Concentration Dependent Effect of Human Dermal Fibroblast Conditioned Medium (DFCM) from Three Various Origins on Keratinocytes Wound Healing.
Biomarkers
Cell Adhesion
/ drug effects
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Cells, Cultured
Culture Media, Conditioned
/ pharmacology
Dermis
/ cytology
Fibroblasts
/ metabolism
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Humans
Keratinocytes
/ physiology
Signal Transduction
Wound Healing
/ drug effects
dermal fibroblast conditioned medium
fibroblasts
keratinocytes
tissue engineering
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Apr 2020
22 Apr 2020
Historique:
received:
13
03
2020
revised:
03
04
2020
accepted:
06
04
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
22
1
2021
Statut:
epublish
Résumé
Fibroblasts secrete many essential factors that can be collected from fibroblast culture medium, which is termed dermal fibroblast conditioned medium (DFCM). Fibroblasts isolated from human skin samples were cultured in vitro using the serum-free keratinocyte-specific medium (Epilife (KM1), or define keratinocytes serum-free medium, DKSFM (KM2) and serum-free fibroblast-specific medium (FM) to collect DFCM-KM1, DFCM-KM2, and DFCM-FM, respectively). We characterised and evaluated the effects of 100-1600 µg/mL DFCM on keratinocytes based on attachment, proliferation, migration and gene expression. Supplementation with 200-400 µg/mL keratinocyte-specific DFCM-KM1 and DFCM-KM2 enhanced the attachment, proliferation and migration of sub-confluent keratinocytes, whereas 200-1600 µg/mL DFCM-FM significantly increased the healing rate in the wound healing assay, and 400-800 µg/mL DFCM-FM was suitable to enhance keratinocyte attachment and proliferation. A real-time (RT
Identifiants
pubmed: 32331278
pii: ijms21082929
doi: 10.3390/ijms21082929
pmc: PMC7215860
pii:
doi:
Substances chimiques
Biomarkers
0
Culture Media, Conditioned
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : SCIENCE FUND (MOSTI)
ID : 02-01-02-SF0964
Organisme : ARUS PERDANA GRANT
ID : AP-2013-015
Organisme : Universiti Kebangsaan Malaysia (UKM) fundamental fund
ID : FF-2015-204
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