Knockout mouse models reveal the contributions of G protein subunits to complement C5a receptor-mediated chemotaxis.
G protein
G protein-coupled receptor (GPCR)
calcium imaging
cell motility
chemotaxis
complement C5a
complement system
gene knockout
immune system
macrophage
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
29 05 2020
29 05 2020
Historique:
received:
19
11
2019
revised:
22
04
2020
pubmed:
26
4
2020
medline:
29
12
2020
entrez:
26
4
2020
Statut:
ppublish
Résumé
G protein-coupled receptor signaling is required for the navigation of immune cells along chemoattractant gradients. However, chemoattractant receptors may couple to more than one type of heterotrimeric G protein, each of which consists of a Gα, Gβ, and Gγ subunit, making it difficult to delineate the critical signaling pathways. Here, we used knockout mouse models and time-lapse microscopy to elucidate Gα and Gβ subunits contributing to complement C5a receptor-mediated chemotaxis. Complement C5a-mediated chemokinesis and chemotaxis were almost completely abolished in macrophages lacking
Identifiants
pubmed: 32332099
pii: S0021-9258(17)49462-2
doi: 10.1074/jbc.RA119.011984
pmc: PMC7261795
doi:
Substances chimiques
Receptor, Anaphylatoxin C5a
0
Heterotrimeric GTP-Binding Proteins
EC 3.6.5.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7726-7742Subventions
Organisme : NCI NIH HHS
ID : R01 CA192381
Pays : United States
Informations de copyright
© 2020 van den Bos et al.
Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
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