RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
27
03
2020
revised:
13
04
2020
accepted:
14
04
2020
pubmed:
26
4
2020
medline:
12
6
2020
entrez:
26
4
2020
Statut:
ppublish
Résumé
The pandemic coronavirus SARS-CoV-2 in the world has caused a large infected population suffering from COVID-19. To curb the spreading of the virus, WHO urgently demanded an extension of screening and testing; thus, a rapid and simple diagnostic method is needed. We applied a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) to achieve the detection of SARS-CoV-2 in 30 min. We designed four sets of LAMP primers (6 primers in each set), targeting the viral RNA of SARS-CoV-2 in the regions of orf1ab, S gene and N gene. A colorimetric change was used to report the results, which enables the outcome of viral RNA amplification to be read by the naked eye without the need of expensive or dedicated instrument. The sensitivity can be 80 copies of viral RNA per ml in a sample. We validated the RT-LAMP method in a hospital in China, employing 16 clinic samples with 8 positives and 8 negatives. The testing results are consistent with the conventional RT-qPCR. In addition, we also show that one-step process without RNA extraction is feasible to achieve RNA amplification directly from a sample. This rapid, simple and sensitive RT-LAMP method paves a way for a large screening at public domain and hospitals, particularly regional hospitals and medical centres in rural areas.
Identifiants
pubmed: 32333644
doi: 10.1111/1751-7915.13586
pmc: PMC7264870
doi:
Substances chimiques
DNA Primers
0
Viral Proteins
0
Banques de données
GENBANK
['NC_045512.2']
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
950-961Subventions
Organisme : Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford.
Pays : International
Informations de copyright
© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
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