Negative charge of the AC-to-Hly linking segment modulates calcium-dependent membrane activities of Bordetella adenylate cyclase toxin.
Adenylate Cyclase Toxin
/ chemistry
Biological Transport
/ genetics
Bordetella pertussis
/ chemistry
Calcium
/ metabolism
Cell Membrane
/ chemistry
Cell Membrane Permeability
/ genetics
Cyclic AMP
/ metabolism
Hemolysis
/ genetics
Humans
Lipid Bilayers
/ chemistry
Protein Conformation, alpha-Helical
/ genetics
AC-to-Hly linking segment
Adenylate cyclase toxin
Calcium dependence
Membrane penetration
NMR structure
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
10
01
2020
revised:
31
03
2020
accepted:
13
04
2020
pubmed:
26
4
2020
medline:
15
12
2020
entrez:
26
4
2020
Statut:
ppublish
Résumé
Two distinct conformers of the adenylate cyclase toxin (CyaA) appear to accomplish its two parallel activities within target cell membrane. The translocating conformer would deliver the N-terminal adenylyl cyclase (AC) enzyme domain across plasma membrane into cytosol of cells, while the pore precursor conformer would assemble into oligomeric cation-selective pores and permeabilize cellular membrane. Both toxin activities then involve a membrane-interacting 'AC-to-Hly-linking segment' (residues 400 to 500). Here, we report the NMR structure of the corresponding CyaA
Identifiants
pubmed: 32333856
pii: S0005-2736(20)30141-3
doi: 10.1016/j.bbamem.2020.183310
pii:
doi:
Substances chimiques
Adenylate Cyclase Toxin
0
Lipid Bilayers
0
Cyclic AMP
E0399OZS9N
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
183310Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.