Reference and Ghost Proteins Identification in Rat C6 Glioma Extracellular Vesicles.
Biochemistry
Cancer
Proteomics
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
22 May 2020
22 May 2020
Historique:
received:
27
02
2020
revised:
23
03
2020
accepted:
03
04
2020
pubmed:
26
4
2020
medline:
26
4
2020
entrez:
26
4
2020
Statut:
ppublish
Résumé
Extracellular vesicles (EVs) mediate intercellular communication and regulate a broad range of biological processes. Novel therapeutic strategies have emerged based on the use of EVs as biological nanoparticles. To separate isolated EVs from protein aggregates and the external part of EVs membrane proteins, we performed a Trypsin/Lys C digestion treatment of EVs pellets, followed by Amicon filtration. After these steps, all the fractions have been subjected to proteomic analyses. Comparison between 6 h Trypsin/Lys C treatment or non-treated EVs revealed a quantitative variation of the surface proteins. Some surface proteins have been demasked after 6 h enzymatic digestion like CD81, CD82, Ust, Vcan, Lamp 1, Rab43, Annexin A2, Synthenin, and VSP37b. Moreover, six ghost proteins have also been identified and one corresponds to a long noncoding RNA. We thus demonstrate the presence of ghost proteins in EVs produced by glioma cells that can contribute to tumorigenesis.
Identifiants
pubmed: 32334413
pii: S2589-0042(20)30230-3
doi: 10.1016/j.isci.2020.101045
pmc: PMC7182720
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101045Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interest.
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