Corneal extracellular matrix decellularization.

Animals Cornea / cytology Deoxyribonucleases / metabolism Extracellular Matrix Octoxynol / chemistry Ribonucleases / metabolism Sodium Dodecyl Sulfate / chemistry Surface-Active Agents / chemistry Swine Tissue Engineering / methods Tissue Scaffolds
Collagen Cornea Extracellular matrix Keratocyte Scaffold Stroma

Journal

Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334

Informations de publication

Date de publication:
2020
Historique:
entrez: 27 4 2020
pubmed: 27 4 2020
medline: 25 5 2021
Statut: ppublish

Résumé

Decellularized corneal scaffolds have the potential to be used as alternatives to donor corneas during keratoplasty. Here a decellularization technique is described that involves the use of sodium dodecyl sulfate, Triton-X100, DNAse and RNAse to remove cells and cellular constituents. We have previously found that this combination of chemicals and enzymes to be effective at removing cells while retaining extracellular matrix proteins. In addition, different methods for assessing if the decellularization process has been successful are discussed. These include techniques to identify and quantify the presence of cells, DNA and extracellular matrix components as well as methods to examine the collagen fibril organization and scaffold transparency.

Identifiants

DOI: 10.1016/bs.mcb.2019.10.013 PMID: 32334721
pubmed: 32334721
pii: S0091-679X(19)30130-X
doi: 10.1016/bs.mcb.2019.10.013
pii:
doi:

Substances chimiques

Surface-Active Agents 0
Sodium Dodecyl Sulfate 368GB5141J
Octoxynol 9002-93-1
Deoxyribonucleases EC 3.1.-
Ribonucleases EC 3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

81-95

Informations de copyright

© 2020 Elsevier Inc. All rights reserved.

Auteurs

Mark Ahearne (M)

Department of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin, University of Dublin, Dublin, Ireland; Trinity Centre for Biomedical Engineering, Trinity Biomedical Science Institute, Trinity College Dublin, University of Dublin, Dublin, Ireland. Electronic address: ahearnm@tcd.ie.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Organes des sens › Oeil › Pôle antérieur du bulbe oculaire › Cornée › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Désoxyribonucléases › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polyéthylène glycols › Octoxinol › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Ribonucléases › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Lipides › Alcools gras › Dodécan-1-ol › Dodécyl-sulfate de sodium › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Tensioactifs › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Corneal extracellular matrix decellularization.
questionsmedicales.fr › Équipement et fournitures › Prothèses et implants › Structures d'échafaudage tissulaires › Corneal extracellular matrix decellularization.