Photodynamic inactivation of methicillin-resistant Staphylococcus aureus on skin using a porphyrinic formulation.


Journal

Photodiagnosis and photodynamic therapy
ISSN: 1873-1597
Titre abrégé: Photodiagnosis Photodyn Ther
Pays: Netherlands
ID NLM: 101226123

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 04 01 2020
revised: 16 03 2020
accepted: 30 03 2020
pubmed: 27 4 2020
medline: 15 5 2021
entrez: 27 4 2020
Statut: ppublish

Résumé

Staphylococcus aureus is responsible for skin and soft tissue infections. Having in mind increased antibiotic resistance, in this study the efficacy of antimicrobial photodynamic therapy (aPDT) with a porphyrinic formulation (FORM) as photosensitizer (PS) to photoinactivate methicillin-resistant Staphylococcus aureus (MRSA) on skin was evaluated. Potassium iodide (KI) and iodopovidone (PVP-I) were also tested in combination with FORM as potentiator agents of FORM efficacy. The aPDT protocol was first developed in Phosphate Buffered Saline (PBS, in vitro). Porcine skin was artificially contaminated with MRSA (ex vivo) and treated with FORM, FORM + KI or FORM + PVP-I under white light. The in vitro results showed that FORM was effective to inactivate MRSA. A substantial reduction in the irradiation time, when compared to FORM alone, was observed for FORM + KI and FORM + PVP-I combinations. On skin, reductions in MRSA survival of 3.1 Log

Identifiants

pubmed: 32335190
pii: S1572-1000(20)30107-1
doi: 10.1016/j.pdpdt.2020.101754
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Photosensitizing Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

101754

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest None.

Auteurs

Márcia Braz (M)

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.

Diana Salvador (D)

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal.

Ana T P C Gomes (ATPC)

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: ana.peixoto@ua.pt.

Mariana Q Mesquita (MQ)

Department of Chemistry and QOPNA & LAQV-REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal.

M Amparo F Faustino (MAF)

Department of Chemistry and QOPNA & LAQV-REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal.

M Graça P M S Neves (MGPMS)

Department of Chemistry and QOPNA & LAQV-REQUIMTE, University of Aveiro, 3810-193 Aveiro, Portugal.

Adelaide Almeida (A)

Department of Biology & CESAM, University of Aveiro, 3810-193 Aveiro, Portugal. Electronic address: aalmeida@ua.pt.

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Classifications MeSH