Tracing incorporation of heavy water into proteins for species-specific metabolic activity in complex communities.

Animals Carbon Isotopes / analysis Deuterium Oxide Humans Isotope Labeling Microbiota Proteins
Heavy water Metabolic activity Metaproteomics Microbiome Protein-SIP

Journal

Journal of proteomics
ISSN: 1876-7737
Titre abrégé: J Proteomics
Pays: Netherlands
ID NLM: 101475056

Informations de publication

Date de publication:
30 06 2020
Historique:
received: 27 01 2020
revised: 08 04 2020
accepted: 19 04 2020
pubmed: 27 4 2020
medline: 22 6 2021
entrez: 27 4 2020
Statut: ppublish

Résumé

Stable isotope probing (SIP) approaches are a suitable tool to identify active organisms in bacterial communities, but adding isotopically labeled substrate can alter both the structure and the functionality of the community. Here, we validated and demonstrated a substrate-independent protein-SIP protocol using isotopically labeled water that captures the entire microbial activity of a community. We found that

Identifiants

DOI: 10.1016/j.jprot.2020.103791 PMID: 32335296
pubmed: 32335296
pii: S1874-3919(20)30159-7
doi: 10.1016/j.jprot.2020.103791
pii:
doi:

Substances chimiques

Carbon Isotopes 0
Proteins 0
Deuterium Oxide J65BV539M3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103791

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests.

Auteurs

Robert Starke (R)

Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany; Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic. Electronic address: robert.starke@biomed.cas.cz.

Kaitlyn Oliphant (K)

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada. Electronic address: koliphan@uoguelph.ca.

Nico Jehmlich (N)

Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany. Electronic address: nico.jehmlich@ufz.de.

Stephanie Serena Schäpe (SS)

Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany. Electronic address: stephanie.schaepe@ufz.de.

Timo Sachsenberg (T)

Applied Bioinformatics, Dept. of Computer Science, University of Tübingen, Tübingen, Germany; Center for Bioinformatics, University of Tübingen, Germany.

Oliver Kohlbacher (O)

Applied Bioinformatics, Dept. of Computer Science, University of Tübingen, Tübingen, Germany; Center for Bioinformatics, University of Tübingen, Germany; Biomolecular Interactions, Max Planck Institute for Developmental Biology, Tübingen, Germany; Institute for Translational Bioinformatics, University Hospital Tübingen, Tübingen, Germany. Electronic address: oliver.kohlbacher@uni-tuebingen.de.

Emma Allen-Vercoe (E)

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada. Electronic address: eav@uoguelph.ca.

Martin von Bergen (M)

Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany; Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany. Electronic address: martin.vonbergen@ufz.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Tracing incorporation of heavy water into...
questionsmedicales.fr › Produits chimiques inorganiques › Isotopes › Isotopes du carbone › Tracing incorporation of heavy water into...
questionsmedicales.fr › Produits chimiques inorganiques › Gaz › Hydrogène › Oxyde de deutérium › Tracing incorporation of heavy water into...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Tracing incorporation of heavy water into...
questionsmedicales.fr › Techniques d'investigation › Marquage isotopique › Tracing incorporation of heavy water into...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Tracing incorporation of heavy water into...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Tracing incorporation of heavy water into...