[Identification of Descarbonsildenafil in an Adulterated Dietary Supplement and Evaluation of Its Inhibitory Activity for Phosphodiesterase Type 5 (PDE5)].
INADEQUATE NMR
X-ray crystal structural analysis
descarbonsildenafil
dietary supplement product
phosphodiesterase type 5 inhibitor
sildenafil
Journal
Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan
ISSN: 0015-6426
Titre abrégé: Shokuhin Eiseigaku Zasshi
Pays: Japan
ID NLM: 0142214
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
4
2020
pubmed:
28
4
2020
medline:
4
8
2020
Statut:
ppublish
Résumé
Some illegal dietary supplements contain phosphodiesterase type 5 (PDE5) inhibitors, such as sildenafil, for exerting "therapeutic" effects in erectile dysfunction. This is apparently dangerous, and thus, should be appropriately regulated. Identification of descarbonsildenafil was first reported in Singapore in a coffee sample labeled to exert male sexual performance enhancement effects. However, it is unclear whether the compound possesses PDE5 inhibitory activity. We encountered during our survey of dietary supplements, a sexual enhancement product commercially available in Tokyo, in which a peak presumed to be of descarbonsildenafil was detected by LC-UV and electrospray ionization-tandem MS (ESI-MS/MS). The compound was isolated and identified as descarbonsildenafil with liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS), NMR, and X-ray crystal structural analysis. In addition, descarbonsildenafil showed PDE5 inhibitory activity in PDE5 inhibition assay, and its IC
Identifiants
pubmed: 32336717
doi: 10.3358/shokueishi.61.34
doi:
Substances chimiques
Phosphodiesterase 5 Inhibitors
0
Sildenafil Citrate
BW9B0ZE037
Cyclic Nucleotide Phosphodiesterases, Type 5
EC 3.1.4.35
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM