Changes in the Radiation Toxicity of Human Lymphoblastic T-cell Line (Jurkat) by a Common Pesticide: Diazinon.
Diazinon
Jurkat Cells
Pesticides
Radiation Tolerance
Radiotherapy
Journal
Journal of biomedical physics & engineering
ISSN: 2251-7200
Titre abrégé: J Biomed Phys Eng
Pays: Iran
ID NLM: 101589641
Informations de publication
Date de publication:
Apr 2020
Apr 2020
Historique:
received:
08
01
2017
accepted:
14
04
2017
entrez:
28
4
2020
pubmed:
28
4
2020
medline:
28
4
2020
Statut:
epublish
Résumé
Diazinon is one of the most common pesticides in the world playing a similar role to radiation and it could cause DNA breaks and genetic effects. In this study, radiosensitivity of a lymphoblastic cell line pretreated by Diazinon was investigated. In this case-control study, the human lymphoblastic T-cell line was divided into 6 groups based on receiving radiation or/and Diazinon. After that, the DNA damage, in all of the groups, were counted by cytokinesis-block micronuclei assay using different indices. The mean frequency of micronuclei, nuclear bridges and nuclear buds in cell groups exposed by both Diazinon and radiation were remarkably higher than the other groups which just received radiation or Diazinon alone. The interaction between radiation and Diazinon treatment was statistically significant for NBUDS index. The results indicated that the Diazinon contamination could affect the radiosensitivity index of cancerous cells while further molecular and in-vivo studies are needed to investigate genetic and toxic effects of Diazinon on DNA and its repair system.
Sections du résumé
BACKGROUND
BACKGROUND
Diazinon is one of the most common pesticides in the world playing a similar role to radiation and it could cause DNA breaks and genetic effects.
OBJECTIVE
OBJECTIVE
In this study, radiosensitivity of a lymphoblastic cell line pretreated by Diazinon was investigated.
MATERIAL AND METHODS
METHODS
In this case-control study, the human lymphoblastic T-cell line was divided into 6 groups based on receiving radiation or/and Diazinon. After that, the DNA damage, in all of the groups, were counted by cytokinesis-block micronuclei assay using different indices.
RESULTS
RESULTS
The mean frequency of micronuclei, nuclear bridges and nuclear buds in cell groups exposed by both Diazinon and radiation were remarkably higher than the other groups which just received radiation or Diazinon alone. The interaction between radiation and Diazinon treatment was statistically significant for NBUDS index.
CONCLUSION
CONCLUSIONS
The results indicated that the Diazinon contamination could affect the radiosensitivity index of cancerous cells while further molecular and in-vivo studies are needed to investigate genetic and toxic effects of Diazinon on DNA and its repair system.
Identifiants
pubmed: 32337181
doi: 10.31661/jbpe.v0i0.715
pii: JBPE-10-2
pmc: PMC7166222
doi:
Types de publication
Journal Article
Langues
eng
Pagination
147-154Informations de copyright
Copyright: © Journal of Biomedical Physics and Engineering.
Références
Iran J Med Sci. 2016 May;41(3):180-5
pubmed: 27217601
Mutat Res. 2003 Jun;543(3):251-72
pubmed: 12787816
Iran J Basic Med Sci. 2014;17(4):303-6
pubmed: 24904724
Duodecim. 2015;131(5):433-40
pubmed: 26237905
BMC Med Phys. 2012 Aug 24;12:3
pubmed: 22920225
Acta Med Okayama. 2015;69(4):189-95
pubmed: 26289909
Am J Epidemiol. 2005 Nov 1;162(9):849-57
pubmed: 16177143
Cancer Epidemiol Biomarkers Prev. 2000 Oct;9(10):1005-15
pubmed: 11045781
Blood. 2012 Jul 12;120(2):366-75
pubmed: 22645179
Int J Mol Cell Med. 2014 Winter;3(1):57-60
pubmed: 24551822
Mutagenesis. 2011 Jan;26(1):125-32
pubmed: 21164193
J Biomed Phys Eng. 2017 Dec 01;7(4):347-354
pubmed: 29445712
Blood. 2006 Aug 15;108(4):1363-9
pubmed: 16621961
Lancet Oncol. 2003 Sep;4(9):529-36
pubmed: 12965273
Methods Mol Biol. 2011;682:217-34
pubmed: 21057931
Mutat Res. 1993 Jan;285(1):35-44
pubmed: 7678131
Genet Mol Biol. 2015 Jul-Sep;38(3):308-15
pubmed: 26500434
Int J Mol Cell Med. 2013 Summer;2(3):131-5
pubmed: 24551803
Arh Hig Rada Toksikol. 2015 Jun;66(2):153-8
pubmed: 26110477
Iran Red Crescent Med J. 2012 Jun;14(6):337-44
pubmed: 22924112
Environ Sci Pollut Res Int. 2016 Feb;23(3):2882-9
pubmed: 26490884
Bull Environ Contam Toxicol. 2011 Sep;87(3):231-7
pubmed: 21681397
Toxicol Lett. 2010 Mar 1;193(1):9-18
pubmed: 19948211
Future Oncol. 2014 Dec;10(15):2359-79
pubmed: 25525845
J Exp Clin Cancer Res. 1999 Jun;18(2):159-66
pubmed: 10464702
Aquat Toxicol. 2011 Mar;102(1-2):114-22
pubmed: 21333264