An antibody-aptamer sandwich cathodic photoelectrochemical biosensor for the detection of progesterone.
Antibody-aptamer sandwich assay
Au–CuO–Cu(2)O
Carbon dots-graphene oxide composites
Cathodic photoelectrochemical biosensor
Progesterone
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Jul 2020
15 Jul 2020
Historique:
received:
06
02
2020
revised:
05
04
2020
accepted:
09
04
2020
pubmed:
28
4
2020
medline:
7
4
2021
entrez:
28
4
2020
Statut:
ppublish
Résumé
The progesterone (P4) level in body fluids can act as an indicator for early pregnancy diagnosis and offers insight into mammalian somatic function. In this work, we designed an antibody-aptamer based sandwich assay as a cathodic photoelectrochemical (PEC) biosensor for P4 detection. The composites of carbon dots and graphene oxide (CDs-GO) with favorable cathodic photocurrent response were used as photoactive materials on which the antibody (Ab) of P4 was immobilized. Meanwhile, high affinity truncated P4 aptamer was immobilized on Au-CuO-Cu
Identifiants
pubmed: 32339148
pii: S0956-5663(20)30207-4
doi: 10.1016/j.bios.2020.112210
pii:
doi:
Substances chimiques
Antibodies, Immobilized
0
Aptamers, Nucleotide
0
graphene oxide
0
Progesterone
4G7DS2Q64Y
Graphite
7782-42-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112210Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.