Impact of taurine on red blood cell metabolism and implications for blood storage.
Journal
Transfusion
ISSN: 1537-2995
Titre abrégé: Transfusion
Pays: United States
ID NLM: 0417360
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
14
01
2020
revised:
18
02
2020
accepted:
22
02
2020
pubmed:
28
4
2020
medline:
22
6
2021
entrez:
28
4
2020
Statut:
ppublish
Résumé
Taurine is an antioxidant that is abundant in some common energy drinks. Here we hypothesized that the antioxidant activity of taurine in red blood cells (RBCs) could be leveraged to counteract storage-induced oxidant stress. Metabolomics analyses were performed on plasma and RBCs from healthy volunteers (n = 4) at baseline and after consumption of a whole can of a common, taurine-rich (1000 mg/serving) energy drink. Reductionistic studies were also performed by incubating human RBCs with taurine ex vivo (unlabeled or Consumption of energy drinks increased plasma and RBC levels of taurine, which was paralleled by increases in glycolysis and glutathione (GSH) metabolism in the RBC. These observations were recapitulated ex vivo after incubation with taurine and hydrogen peroxide. Taurine levels in the RBCs from the REDS-III RBC-Omics donor biobank were directly proportional to the total levels of GSH and glutathionylated metabolites and inversely correlated to oxidative hemolysis measurements. Storage of human RBCs in the presence of taurine improved energy and redox markers of storage quality and increased posttransfusion recoveries in FVB mice. Taurine modulates RBC antioxidant metabolism in vivo and ex vivo, an observation of potential relevance to transfusion medicine.
Sections du résumé
BACKGROUND
Taurine is an antioxidant that is abundant in some common energy drinks. Here we hypothesized that the antioxidant activity of taurine in red blood cells (RBCs) could be leveraged to counteract storage-induced oxidant stress.
STUDY DESIGN AND METHODS
Metabolomics analyses were performed on plasma and RBCs from healthy volunteers (n = 4) at baseline and after consumption of a whole can of a common, taurine-rich (1000 mg/serving) energy drink. Reductionistic studies were also performed by incubating human RBCs with taurine ex vivo (unlabeled or
RESULTS
Consumption of energy drinks increased plasma and RBC levels of taurine, which was paralleled by increases in glycolysis and glutathione (GSH) metabolism in the RBC. These observations were recapitulated ex vivo after incubation with taurine and hydrogen peroxide. Taurine levels in the RBCs from the REDS-III RBC-Omics donor biobank were directly proportional to the total levels of GSH and glutathionylated metabolites and inversely correlated to oxidative hemolysis measurements. Storage of human RBCs in the presence of taurine improved energy and redox markers of storage quality and increased posttransfusion recoveries in FVB mice.
CONCLUSION
Taurine modulates RBC antioxidant metabolism in vivo and ex vivo, an observation of potential relevance to transfusion medicine.
Identifiants
pubmed: 32339326
doi: 10.1111/trf.15810
pmc: PMC7995806
mid: NIHMS1676414
doi:
Substances chimiques
Taurine
1EQV5MLY3D
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1212-1226Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL146442
Pays : United States
Organisme : NHLBI NIH HHS
ID : HHSN268201100001I
Pays : United States
Organisme : NIGMS NIH HHS
ID : RM1 GM131968
Pays : United States
Organisme : NIGMS NIH HHS
ID : RM1GM131968
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01HL146442
Pays : United States
Organisme : NIH HHS
ID : S10 OD021641
Pays : United States
Organisme : NHLBI NIH HHS
ID : HHSN2682011
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01HL148151
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL148151
Pays : United States
Organisme : NHLBI NIH HHS
ID : R21 HL150032
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL149714
Pays : United States
Organisme : WHI NIH HHS
ID : HHSN268201100001C
Pays : United States
Informations de copyright
© 2020 AABB.
Références
Transfusion. 2019 Jul;59(7):2264-2275
pubmed: 31002399
Transfusion. 2016 Oct;56(10):2560-2570
pubmed: 27514704
Nucleic Acids Res. 2018 Jul 2;46(W1):W514-W522
pubmed: 29878180
Free Radic Biol Med. 1994 Jun;16(6):703-12
pubmed: 8070673
Biol Trace Elem Res. 2017 Feb;175(2):388-395
pubmed: 27334436
Birth Defects Res. 2017 Dec 1;109(20):1640-1648
pubmed: 29251842
Transfusion. 2011 Jul;51(7):1439-49
pubmed: 21276001
Blood Transfus. 2014 Jul;12(3):376-87
pubmed: 25074788
Transfusion. 2019 May;59(5):1620-1623
pubmed: 30865302
Haematologica. 2018 Feb;103(2):361-372
pubmed: 29079593
Transfusion. 2019 Jan;59(1):89-100
pubmed: 30353560
Haematologica. 2012 Jan;97(1):107-15
pubmed: 21993682
Free Radic Biol Med. 2016 Jul;96:152-65
pubmed: 27094493
J Histochem Cytochem. 2009 Nov;57(11):1003-11
pubmed: 19546473
Am J Clin Nutr. 1988 Apr;47(4):660-3
pubmed: 3354491
Blood. 2016 Sep 29;128(13):e43-50
pubmed: 27554084
Blood Adv. 2017 Jun 27;1(15):1132-1141
pubmed: 29034365
Front Med (Lausanne). 2018 May 15;5:130
pubmed: 29868587
J Cell Mol Med. 2007 Jan-Feb;11(1):148-55
pubmed: 17367509
Environ Sci Pollut Res Int. 2017 Aug;24(23):19086-19097
pubmed: 28660510
Adv Exp Med Biol. 2003;526:527-36
pubmed: 12908639
Can J Physiol Pharmacol. 2009 Feb;87(2):91-9
pubmed: 19234572
Transfusion. 2016 Feb;56(2):392-403
pubmed: 26477888
Transfusion. 2002 Jun;42(6):747-52
pubmed: 12147028
Transfusion. 2015 Jan;55(1):205-19
pubmed: 25130459
Transfusion. 2015 Jun;55(6):1155-68
pubmed: 25556331
Transfusion. 2014 Jan;54(1):137-48
pubmed: 23721209
Transfusion. 2017 Feb;57(2):325-336
pubmed: 27813142
Blood. 2016 Sep 22;128(12):e32-42
pubmed: 27405778
Transfusion. 2016 Apr;56(4):980-93
pubmed: 26662506
Expert Rev Proteomics. 2018 Nov;15(11):855-864
pubmed: 30278801
Pathophysiology. 2012 Apr;19(2):137-48
pubmed: 22626456
J Mol Biol. 2018 Sep 14;430(18 Pt B):3234-3250
pubmed: 29932944
Transfusion. 2017 Nov;57(11):2657-2664
pubmed: 28921569
Haematologica. 2016 May;101(5):578-86
pubmed: 26921359
J Proteome Res. 2017 Aug 4;16(8):2752-2761
pubmed: 28689405
BMB Rep. 2008 Sep 30;41(9):657-63
pubmed: 18823590
Transfusion. 2018 Aug;58(8):1992-2002
pubmed: 29624679
Adv Exp Med Biol. 1998;442:163-8
pubmed: 9635028
Rapid Commun Mass Spectrom. 2017 Apr 30;31(8):663-673
pubmed: 28195377
Vox Sang. 2015 Aug;109(2):148-54
pubmed: 26053689
Amino Acids. 2012 Jun;42(6):2223-32
pubmed: 21691752
Amino Acids. 2008 Feb;34(2):321-8
pubmed: 16955229
Blood Adv. 2019 Aug 13;3(15):2272-2285
pubmed: 31350307
Biol Pharm Bull. 1993 Oct;16(10):970-2
pubmed: 8287047
Transfusion. 2019 Jan;59(1):57-66
pubmed: 30566231
Am J Dis Child. 1988 Nov;142(11):1194-8
pubmed: 3177325
Regul Toxicol Pharmacol. 2008 Apr;50(3):376-99
pubmed: 18325648
Adv Exp Med Biol. 2000;483:411-29
pubmed: 11787627
Nucleic Acids Res. 2018 Jul 2;46(W1):W486-W494
pubmed: 29762782
Clin Chim Acta. 2006 Apr;366(1-2):190-5
pubmed: 16325165
Transfusion. 2016 Apr;56(4):852-62
pubmed: 26749434
Blood Transfus. 2019 Jul;17(4):281-288
pubmed: 31184577
Transfusion. 2018 Dec;58(12):2978-2991
pubmed: 30312994
Transfusion. 2019 Jan;59(1):79-88
pubmed: 30408207
Transfusion. 2019 Jan;59(1):46-56
pubmed: 30267427
Mol Vis. 2012;18:2673-86
pubmed: 23170060
Vox Sang. 2013 Nov;105(4):271-82
pubmed: 23815264
Methods Mol Biol. 2019;1978:121-135
pubmed: 31119660
J Clin Invest. 2017 Jan 3;127(1):375-382
pubmed: 27941245
Adv Exp Med Biol. 2000;483:503-22
pubmed: 11787637
Methods Mol Biol. 2019;1978:13-26
pubmed: 31119654