Quantification of native mRNA dynamics in living neurons using fluorescence correlation spectroscopy and reduction-triggered fluorescent probes.
RNA transport
cellular dynamics
fluorescence correlation spectroscopy (FCS)
gene regulation
hippocampus
live-cell imaging
mRNA
neuron
reduction-triggered fluorescence (RETF)
transcription
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
05 06 2020
05 06 2020
Historique:
received:
02
09
2019
revised:
22
04
2020
pubmed:
29
4
2020
medline:
29
12
2020
entrez:
29
4
2020
Statut:
ppublish
Résumé
RNA localization in subcellular compartments is essential for spatial and temporal regulation of protein expression in neurons. Several techniques have been developed to visualize mRNAs inside cells, but the study of the behavior of endogenous and nonengineered mRNAs in living neurons has just started. In this study, we combined reduction-triggered fluorescent (RETF) probes and fluorescence correlation spectroscopy (FCS) to investigate the diffusion properties of activity-regulated cytoskeleton-associated protein (
Identifiants
pubmed: 32341124
pii: S0021-9258(17)49433-6
doi: 10.1074/jbc.RA119.010921
pmc: PMC7278347
doi:
Substances chimiques
Cytoskeletal Proteins
0
Fluorescent Dyes
0
Inositol 1,4,5-Trisphosphate Receptors
0
Itpr1 protein, rat
0
Nerve Tissue Proteins
0
RNA, Messenger
0
activity regulated cytoskeletal-associated protein
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7923-7940Informations de copyright
© 2020 Fujita et al.
Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Références
Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):E9697-E9706
pubmed: 30254174
J Comp Neurol. 2012 Oct 1;520(14):3105-19
pubmed: 22350812
Nat Biotechnol. 1996 Mar;14(3):303-8
pubmed: 9630890
J Neurosci. 1998 Jan 1;18(1):26-35
pubmed: 9412483
J Neurosci. 2006 Jul 5;26(27):7139-42
pubmed: 16822968
Neuron. 2006 Nov 9;52(3):445-59
pubmed: 17088211
Cell. 2018 Jan 11;172(1-2):275-288.e18
pubmed: 29328916
Microscopy (Oxf). 2018 Jun 1;67(3):129-143
pubmed: 29554288
Bioconjug Chem. 2008 Jun;19(6):1219-26
pubmed: 18476727
J Comp Neurol. 2007 Jan 20;500(3):433-47
pubmed: 17120280
Curr Opin Neurobiol. 2017 Aug;45:169-177
pubmed: 28633045
Science. 1998 Apr 24;280(5363):585-90
pubmed: 9554849
Neuron. 2009 Jun 11;62(5):670-82
pubmed: 19524526
Nucleic Acids Res. 2002 Nov 1;30(21):e122
pubmed: 12409481
Neurosci Res. 2018 Nov;136:13-32
pubmed: 29395358
Angew Chem Int Ed Engl. 2011 Dec 9;50(50):12020-3
pubmed: 22021091
Curr Biol. 2003 Jan 21;13(2):161-167
pubmed: 12546792
Nat Neurosci. 1999 Dec;2(12):1120-4
pubmed: 10570490
Neuron. 1995 Feb;14(2):433-45
pubmed: 7857651
Nat Methods. 2007 May;4(5):413-9
pubmed: 17401370
Neuroscience. 2004;125(1):7-11
pubmed: 15051140
Exp Neurol. 1959 Nov;1:491-527
pubmed: 14435979
EMBO J. 2011 Aug 31;30(17):3540-52
pubmed: 21878995
Biophys J. 2000 Dec;79(6):3294-306
pubmed: 11106632
Cell. 2016 Apr 7;165(2):488-96
pubmed: 26997482
Nat Methods. 2008 Oct;5(10):877-9
pubmed: 18806792
J Neurosci. 2014 Mar 26;34(13):4481-93
pubmed: 24671994
Development. 2012 Sep;139(18):3263-76
pubmed: 22912410
J Biol Chem. 2013 Jul 12;288(28):20361-8
pubmed: 23720759
PLoS One. 2015 May 28;10(5):e0128241
pubmed: 26020927
Nat Struct Mol Biol. 2019 Jul;26(7):557-566
pubmed: 31270476
Brain Res. 2001 May 18;901(1-2):237-46
pubmed: 11368972
Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):17008-13
pubmed: 16284251
Cell. 2015 Jul 2;162(1):211-20
pubmed: 26140598
Biophys J. 2002 Jul;83(1):510-22
pubmed: 12080138
Proc Natl Acad Sci U S A. 1995 Jun 6;92(12):5734-8
pubmed: 7777577
Bioconjug Chem. 2009 May 20;20(5):1026-36
pubmed: 19374406
Biophys J. 2012 Jun 20;102(12):2936-44
pubmed: 22735544
Curr Opin Neurobiol. 2001 Oct;11(5):558-63
pubmed: 11595488
EMBO Rep. 2011 Sep 30;12(10):1077-84
pubmed: 21869818
J Neurosci. 1998 Jul 15;18(14):5366-73
pubmed: 9651219
Receptors Channels. 1993;1(1):11-24
pubmed: 8081710
Nat Neurosci. 2006 Jul;9(7):887-95
pubmed: 16732277
Biophys J. 2001 Jun;80(6):2987-99
pubmed: 11371471
RNA Biol. 2014;11(8):1083-95
pubmed: 25483044
Traffic. 2011 Aug;12(8):1000-11
pubmed: 21518164
Science. 2014 Jan 24;343(6169):422-4
pubmed: 24458643
Nat Neurosci. 2011 May 29;14(7):848-56
pubmed: 21623364
Mol Cell. 1998 Oct;2(4):437-45
pubmed: 9809065
Learn Mem. 2000 Sep-Oct;7(5):312-20
pubmed: 11040263
Cell. 2018 Jan 11;172(1-2):262-274.e11
pubmed: 29328915
J Phys Chem A. 2013 Jan 10;117(1):27-33
pubmed: 23210598
Neuron. 2006 Sep 21;51(6):685-90
pubmed: 16982415
Sci Adv. 2018 Jun 20;4(6):eaar3448
pubmed: 29938222
PLoS Biol. 2006 Oct;4(10):e309
pubmed: 17048983
Biophys J. 2006 Nov 15;91(10):3921-36
pubmed: 16950841
Sci Rep. 2016 Dec 13;6:38910
pubmed: 27958374
Biophys J. 2002 Jan;82(1 Pt 1):366-77
pubmed: 11751323
J Biol Chem. 2004 Dec 17;279(51):53427-34
pubmed: 15475564
J Mol Biol. 2000 May 12;298(4):677-89
pubmed: 10788329
J Biol Chem. 2010 Apr 23;285(17):13142-53
pubmed: 20185826
Nat Rev Neurosci. 2007 Oct;8(10):776-89
pubmed: 17848965