Measuring cell displacements in opaque tissues: dynamic light scattering in the multiple scattering regime.


Journal

Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630

Informations de publication

Date de publication:
01 Apr 2020
Historique:
received: 21 01 2020
revised: 13 03 2020
accepted: 25 03 2020
entrez: 29 4 2020
pubmed: 29 4 2020
medline: 29 4 2020
Statut: epublish

Résumé

Coherent light scattered by tissues brings structural and dynamic information, at depth, that standard imaging techniques cannot reach. Dynamics of cells or sub-cellular elements can be measured thanks to dynamic light scattering in thin samples (single scattering regime) or thanks to diffusive wave spectroscopy in thick samples (diffusion regime). Here, we address the intermediate regime and provide an analytical relationship between scattered light fluctuations and the distribution of cell displacements as a function of time. We illustrate our method by characterizing cell motility inside half millimeter thick multicellular aggregates.

Identifiants

pubmed: 32341883
doi: 10.1364/BOE.388360
pii: 388360
pmc: PMC7173902
doi:

Types de publication

Journal Article

Langues

eng

Pagination

2277-2297

Informations de copyright

© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.

Déclaration de conflit d'intérêts

The authors declares no conflict of interest.

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Auteurs

Benjamin Brunel (B)

Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, CNRS, F-38000 Grenoble, France.

Vincent Levy (V)

Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, CNRS, F-38000 Grenoble, France.

Arnaud Millet (A)

Institute for Advanced Biosciences, Inserm U1209 - CNRS UMR 5309, Université Grenoble Alpes, F-38000 Grenoble, France.
Research Department, University Hospital of Grenoble Alpes, F-38000 Grenoble, France.

Monika Elzbieta Dolega (ME)

Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, CNRS, F-38000 Grenoble, France.

Antoine Delon (A)

Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, CNRS, F-38000 Grenoble, France.

Romain Pierrat (R)

ESPCI Paris, PSL University, CNRS, Institut Langevin, 1 rue Jussieu, F-75005, Paris, France.

Giovanni Cappello (G)

Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, CNRS, F-38000 Grenoble, France.

Classifications MeSH