Expression of 1B capsid protein of Foot-and-mouth disease virus (FMDV) using baculovirus expression system and its validation in detecting SAT 2- specific antisera.

Baculovirus expression system Capsid protein Foot and Mouth Disease Protein expression

Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2020
Historique:
received: 28 01 2020
accepted: 19 03 2020
entrez: 29 4 2020
pubmed: 29 4 2020
medline: 29 4 2020
Statut: epublish

Résumé

Foot-and-mouth disease virus (FMDV) is one of the most devastating animal viruses that affect livestock worldwide. The 1B capsid of FMDV has been widely used to detect and confirm the infection. In the present study, the sequence coding for 1B subunit of FMDV capsid was expressed in insect cells using the baculovirus expression system under the polyhedrin (

Identifiants

pubmed: 32341896
doi: 10.7717/peerj.8946
pii: 8946
pmc: PMC7182021
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e8946

Informations de copyright

©2020 Elmenofy et al.

Déclaration de conflit d'intérêts

The authors declare that there are no competing interests.

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Auteurs

Wael Elmenofy (W)

Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.

Ismail Mohamed (I)

Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.

Lamiaa El-Gaied (L)

Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.

Reda Salem (R)

Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.

Gamal Osman (G)

Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt.
Department of Biology, Umm Al-Qura University, Makkah, Makkah, Saudi Arabia.
Research Laboratories Center, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia.

Mohamed Ibrahim (M)

Department of Molecular and Cell Biology, University of Texas at Dallas, Richardson, Texas, USA.

Classifications MeSH