A delayed fractionated dose RTS,S AS01 vaccine regimen mediates protection via improved T follicular helper and B cell responses.


Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
29 04 2020
Historique:
received: 15 09 2019
accepted: 14 04 2020
pubmed: 29 4 2020
medline: 10 4 2021
entrez: 29 4 2020
Statut: epublish

Résumé

Malaria-071, a controlled human malaria infection trial, demonstrated that administration of three doses of RTS,S/AS01 malaria vaccine given at one-month intervals was inferior to a delayed fractional dose (DFD) schedule (62.5% vs 86.7% protection, respectively). To investigate the underlying immunologic mechanism, we analyzed the B and T peripheral follicular helper cell (pTfh) responses. Here, we show that protection in both study arms was associated with early induction of functional IL-21-secreting circumsporozoite (CSP)-specific pTfh cells, together with induction of CSP-specific memory B cell responses after the second dose that persisted after the third dose. Data integration of key immunologic measures identified a subset of non-protected individuals in the standard (STD) vaccine arm who lost prior protective B cell responses after receiving the third vaccine dose. We conclude that the DFD regimen favors persistence of functional B cells after the third dose.

Identifiants

pubmed: 32342859
doi: 10.7554/eLife.51889
pii: 51889
pmc: PMC7213985
doi:
pii:

Substances chimiques

Antibodies, Protozoan 0
Interleukins 0
Malaria Vaccines 0
interleukin-21 MKM3CA6LT1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

SP, SC, PL, LP, UW, SP No competing interests declared, EJ is an employee of GSK, and owns shares of GSK

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Auteurs

Suresh Pallikkuth (S)

Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, United States.

Sidhartha Chaudhury (S)

Biotechnology HPC Software Applications Institute, Telemedicine and Advanced Technology Research Center, U.S. Army Medical Research and Development Command, Fort Detrick, United States.

Pinyi Lu (P)

Biotechnology HPC Software Applications Institute, Telemedicine and Advanced Technology Research Center, U.S. Army Medical Research and Development Command, Fort Detrick, United States.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, United States.

Li Pan (L)

Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, United States.

Erik Jongert (E)

GSK Vaccine, Rixensart, Belgium.

Ulrike Wille-Reece (U)

PATH's Malaria Vaccine Initiative, Washington DC, United States.

Savita Pahwa (S)

Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, United States.

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