Expression of lamin C2 in mammalian oocytes.
Animals
Female
Gene Expression Regulation, Developmental
/ genetics
Germ Cells
/ growth & development
Laminin
/ genetics
Male
Meiosis
/ genetics
Mice
Mice, Knockout
Nuclear Envelope
/ genetics
Oocytes
/ growth & development
Oogenesis
/ genetics
Ovary
/ growth & development
RNA, Messenger
/ genetics
Spermatocytes
/ growth & development
Testis
/ growth & development
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
12
2019
accepted:
13
02
2020
entrez:
29
4
2020
pubmed:
29
4
2020
medline:
2
7
2020
Statut:
epublish
Résumé
Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.
Identifiants
pubmed: 32343699
doi: 10.1371/journal.pone.0229781
pii: PONE-D-19-34378
pmc: PMC7188254
doi:
Substances chimiques
Laminin
0
RNA, Messenger
0
lamin C2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0229781Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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