Brevinin-1GHd: a novel Hylarana guentheri skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential.
Amphibian Proteins
/ chemistry
Animals
Anti-Infective Agents
/ chemistry
Antineoplastic Agents
/ chemistry
Bacteria
/ drug effects
Biofilms
/ drug effects
Candida albicans
/ drug effects
Cell Proliferation
/ drug effects
Dose-Response Relationship, Drug
Humans
Kinetics
Neoplasms
/ drug therapy
PC-3 Cells
Protein Conformation
Ranidae
Structure-Activity Relationship
Brevinin-1GHd
anti-proliferation
antimicrobial peptides (AMPs)
biofilms
resistant strains
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
29 05 2020
29 05 2020
Historique:
received:
09
01
2020
revised:
02
04
2020
accepted:
20
04
2020
pubmed:
30
4
2020
medline:
24
3
2021
entrez:
30
4
2020
Statut:
ppublish
Résumé
Host-defense antimicrobial peptides (AMPs) from amphibians are usually considered as one of the most promising next-generation antibiotics because of their excellent antimicrobial properties and low cytotoxicity. In the present study, one novel Brevinin-1 type peptide, Brevinin-1GHd, was isolated and characterized from the skin secretion of the frog, Hylarana guentheri. Brevinin-1GHd was found to possess a wide range of antimicrobial activity through penetrating the bacterial membrane within a short time while showing low hemolysis at bactericidal concentrations, even against the resistant strains. It also inhibited and eradicated biofilms that are thought to be closely related to the rise in resistance. Meanwhile, Brevinin-1GHd exhibited wide-spectrum anti-proliferation activity toward human cancer lines. Taken together, these results indicate that Brevinin-1GHd with its excellent antimicrobial and anticancer activities is a promising candidate for a novel antibiotic agent, and study of its structure-activity relationships also provided a rational template for further research and peptide analog design.
Identifiants
pubmed: 32347293
pii: 222871
doi: 10.1042/BSR20200019
pmc: PMC7225409
pii:
doi:
Substances chimiques
Amphibian Proteins
0
Anti-Infective Agents
0
Antineoplastic Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 The Author(s).
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